Electrical excitability of neuronal networks based on the voltage threshold of electrical stimulation

Microelectrode arrays (MEAs) have been widely used in studies on the electrophysiological features of neuronal networks. In classic MEA experiments, spike or burst rates and spike waveforms are the primary characteristics used to evaluate the neuronal network excitability. Here, we introduced a new...

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Bibliographic Details
Published in:Scientific reports 2024-12, Vol.14 (1), p.31573-11, Article 31573
Main Authors: Meng, Chen, Lu, Yang, Huang, Yan, Lü, Xiaoying
Format: Article
Language:English
Subjects:
631/154/1435/2163
631/378/1697/2601
Action Potentials - drug effects
Action Potentials - physiology
Animals
Brain slice preparation
Data analysis
Drug screening
Electric Stimulation - methods
Electrical stimuli
Electrodes
Excitability
Experiments
Firing pattern
Humanities and Social Sciences
Microelectrodes
multidisciplinary
Neocortex - physiology
Nerve Net - drug effects
Nerve Net - physiology
Neural networks
Neurons - physiology
Nickel - pharmacology
Rats
Research methodology
Science
Science (multidisciplinary)
Voltage
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Description
Summary:Microelectrode arrays (MEAs) have been widely used in studies on the electrophysiological features of neuronal networks. In classic MEA experiments, spike or burst rates and spike waveforms are the primary characteristics used to evaluate the neuronal network excitability. Here, we introduced a new method to assess the excitability using the voltage threshold of electrical stimulation. We tested the stability of the voltage threshold during the experiment and demonstrated the reliability of our method by examining the effect of Ni 2+ on neocortical neuronal networks of acute brain slices from rats. Moreover, we compared our new method with the spontaneous activity analysis, which is one of the most commonly used methods in protocols for large-scale drug screening with MEA; our new method performed better in the experiments investigating the neocortical neuronal network excitability after the application of Ni 2+ . Based on the results from our study, our new method has great potential for use in large-scale screening of drugs.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-78142-7