A novel glutathione-S-transferase-based biosensor for pyrethroid insecticides: From inhibition study to detection

• Published in: Sensors and actuators reports 2022-11, Vol.4, p.100093, Article 100093
• Main Authors: Ribeiro, Eliane Braga, Ribeiro, Danilo Braga, dos Santos Soares, Alexandra Martins, Marques, Paulo Roberto B.O., Badea, Mihaela, Targa, Marcelo, Granato, José Aparecido, Nunes, Gilvanda Silva
• Format: Article
• Language: English
• Subjects: GST inhibition; Molecular docking; Novel GST-based biosensor; Pyrethroid insecticides
• ISSN: 2666-0539; 2666-0539
• DOI: 10.1016/j.snr.2022.100093

•In silico studies indicated binding affinity of pyrethroid insecticides to the glutathione S-transferase-GST catalytic site.•Also, the selected pyrethroid insecticides caused in vitro inhibition of GST.•The GST-based biosensor showed a higher sensitivity for tetramethrin, bifenthrin, lambda-cyhalothrin and deltamethrin. In this study, the inhibitory potential of four pyrethroid compounds on glutathione S-transferase (GST) by using molecular docking and in vitro assays was evaluated. Docking simulations of the pyrethroid ligands (bifenthrin, deltamethrin, lambda-cyhalothrin and tetramethrin) on the active site of GST were performed using Molegro Virtual Docker-MVD, according to the re-docking protocol. All tested compounds had a negative binding energy corresponding to a MolDock score ranging from -100.9 to -84.6. In the colorimetric in vitro assays, inhibition constants, Ki, varying from 0.29 to 20.0 µmol L−1 and IC50 values varying from 1.3 to 3.3 mg L−1 were obtained. These preliminary results indicated the great affinity of the selected compounds to the catalytic site of the GST enzyme, allowing thus the development of a GST-based screen-printed electrochemical biosensor modified with Meldola's blue-Reinecke's salt (MBRS) mediator. High sensitivity (LOD of 0.9, 1.6, 3.6 and 9.5 μg L−1, for tetramethrin, bifenthrin, λ-cyhalothrin, and deltamethrin, respectively), and also good accuracy (recoveries into the acceptable range) were observed for the selected pesticides. The electrochemical device developed herein proved to be suitable for assessing pyrethroid compounds in water and agricultural crops, and displayed good reproducibility and stability over time. [Display omitted]