The Therapeutic Potential of Intra-Articular Injection of Synthetic Deer Antler Peptides in a Rat Model of Knee Osteoarthritis

• Published in: International journal of molecular sciences 2024-06, Vol.25 (11), p.6041
• Main Authors: Hung, Yu-Chou, Chen, Li-Jin, Wang, Jen-Hung, Ho, Tsung-Jung, Tseng, Guo-Fang, Chen, Hao-Ping
• Format: Article
• Language: English
• Subjects: Animals; Antiarthritic agents; Antlers - chemistry; Arthritis; Care and treatment; Cartilage; Cartilage, Articular - drug effects; Cartilage, Articular - metabolism; Cartilage, Articular - pathology; Chinese medicine; chondroprotection; Collagen; Collagenases; Deer; deer antler; Disease Models, Animal; Exercise; Health aspects; Hyaluronic acid; Hyaluronic Acid - administration & dosage; Injections, Intra-Articular; joint; Knee; kyotorphin; Male; neokyotorphin; Nonsteroidal anti-inflammatory drugs; Osteoarthritis; Osteoarthritis, Knee - chemically induced; Osteoarthritis, Knee - drug therapy; Osteoarthritis, Knee - pathology; Pain; Peptides; Peptides - administration & dosage; Peptides - pharmacology; Peptides - therapeutic use; Physical fitness; Rats; Rats, Sprague-Dawley
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25116041

Synthetic deer antler peptides (TSKYR, TSK, and YR) stimulate the proliferation of human chondrocytes and osteoblasts and increase the chondrocyte content of collagen and glycosamino-glycan in vitro. This study investigated the peptide mixture's pain relief and chondroprotective effect in a rat model of collagenase-induced osteoarthritis. Thirty-six adult male Sprague-Dawley rats were divided into three groups: control (saline), positive control (hyaluronic acid), and ex-perimental (peptides). Intra-articular collagenase injections were administered on days 1 and 4 to induce osteoarthritis in the left knees of the rats. Two injections of saline, hyaluronic acid, or the peptides were injected into the same knees of each corresponding group at the beginning of week one and two, respectively. Joint swelling, arthritic pain, and histopathological changes were evaluated. Injection of the peptides significantly reduced arthritic pain compared to the control group, as evidenced by the closer-to-normal weight-bearing and paw withdrawal threshold test results. Histological analyses showed reduced cartilage matrix loss and improved total cartilage degeneration score in the experimental versus the control group. Our findings suggest that intra-articular injection of synthetic deer antler peptides is a promising treatment for osteoarthritis.