Al-Tolerant Barley Mutant hvatr.g Shows the ATR-Regulated DNA Damage Response to Maleic Acid Hydrazide

ATR, a DNA damage signaling kinase, is required for cell cycle checkpoint regulation and detecting DNA damage caused by genotoxic factors including Al ions. We analyzed the function of the gene in response to chemical clastogen-maleic acid hydrazide (MH). For this purpose, the Al-tolerant barley TIL...

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Bibliographic Details
Published in:International journal of molecular sciences 2020-11, Vol.21 (22), p.8500
Main Authors: Jaskowiak, Joanna, Kwasniewska, Jolanta, Szurman-Zubrzycka, Miriam, Rojek-Jelonek, Magdalena, Larsen, Paul B, Szarejko, Iwona
Format: Article
Language:English
Subjects:
Aluminum
Aluminum - pharmacology
aluminum toxicity
Arabidopsis Proteins - metabolism
Ataxia
Ataxia Telangiectasia Mutated Proteins - metabolism
ATR
Barley
Cell cycle
Cell Cycle - drug effects
Cell Cycle - genetics
Cell Nucleus - drug effects
Cell Nucleus - genetics
Chromosomes
Damage detection
Damage tolerance
DDR pathway
Deoxyribonucleic acid
DNA
DNA damage
DNA Damage - drug effects
DNA Damage - genetics
DNA repair
Flow cytometry
Gene expression
Genome, Plant - drug effects
Genome, Plant - genetics
Genomes
Genotoxicity
Genotype
Genotypes
Hordeum - drug effects
Hordeum - genetics
Kinases
Maleic acid
Maleic Hydrazide - pharmacology
Micronuclei
Micronuclei, Chromosome-Defective - drug effects
Mutagens - pharmacology
Mutants
Mutation
Mutation - drug effects
Mutation - genetics
Plant Roots - drug effects
Plant Roots - genetics
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Summary:ATR, a DNA damage signaling kinase, is required for cell cycle checkpoint regulation and detecting DNA damage caused by genotoxic factors including Al ions. We analyzed the function of the gene in response to chemical clastogen-maleic acid hydrazide (MH). For this purpose, the Al-tolerant barley TILLING mutant was used. We described the effects of MH on the nuclear genome of mutant and its WT parent cv. "Sebastian", showing that the genotoxic effect measured by TUNEL test and frequency of cells with micronuclei was much stronger in than in WT. MH caused a significant decrease in the mitotic activity of root cells in both genotypes, however this effect was significantly stronger in "Sebastian". The impact of MH on the roots cell cycle, analyzed using flow cytometry, showed no differences between the mutant and WT.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms21228500