Genetic Evaluation of a Wildtype Population of Cornus mas Accessions in Austria

• Published in: Diversity (Basel) 2023-10, Vol.15 (10), p.1031
• Main Authors: Borroto Fernandez, Eduviges G., Khayatzadeh, Negar, Mészáros, Gábor, Fink, Simon, Hanzer, Veronika, Sölkner, Johann, Laimer, Margit
• Format: Article
• Language: English
• Subjects: admixture; Biodiversity; Breeding; Cherries; Cornelian cherry; Cornus mas; Diploids; Drought; Fruits; Genetic diversity; Genetic relationship; Heterozygosity; Mesolithic; population structure; SSRs; Subpopulations
• ISSN: 1424-2818; 1424-2818
• DOI: 10.3390/d15101031

Cornus mas grows in the “Cornelian Cherry Valley” in Lower Austria. The characterization of the biodiversity of this underutilized crop was undertaken to increase its visibility and to develop breeding parameters. To determine the genetic relationships among Austrian Cornelian cherries, a collection of 447 mainly wild accessions originating from four regions in Lower Austria were genotyped. Analyses of seven microsatellite loci detected a total of 74 alleles, with 10.57 being the average number per locus. Shannon’s index was calculated to be 0.95 in locus CM008 and 1.80 in locus CM020. The average values of observed and expected heterozygosity were 0.54 ± 0.11 and 0.70 ± 0.12. The analyses revealed a high level of diversity. All accessions could be distinguished from each other, and wild and cultivated accessions could be separated. The pattern of grouping of the accessions based on the STRUCTURE (version 2.3.4) analyses suggested a greater number of subpopulations (K = 9). Karyotyping consistently yielded diploid chromosome numbers 2n = 2x = 18 for a millennial accession. The evaluations yielded a high degree of diversity, which provides excellent starting conditions for considerations of breeding strategies for the genetic enhancement of C. mas in Austria.