Genetic Diversity Analysis of Exon-2 Growth Hormone (GH) Gene in Crossbred Chicken (Sentul X Arab) using PCR-RFLP Technique

The research aims to analyze the genetic diversity of the GH gene using the PCR-RFLP technique. Fifty blood samples were collected from crossed Sentul and Arab Chickens aged three months. DNA was extracted from whole blood samples, and its quantity was evaluated by bio-drop technique. Extracted DNA...

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Bibliographic Details
Published in:BIO web of conferences 2024-01, Vol.88, p.6
Main Authors: IrmayaH, Dika, Depison, Ardiantoro, Ari, Suyadi
Format: Article
Language:English
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Summary:The research aims to analyze the genetic diversity of the GH gene using the PCR-RFLP technique. Fifty blood samples were collected from crossed Sentul and Arab Chickens aged three months. DNA was extracted from whole blood samples, and its quantity was evaluated by bio-drop technique. Extracted DNA was amplified for gene GH using a PCR machine. The PCR product was restricted by MspI (C↓CGG) and Hae-III (GG↓CC) enzymes for identifying the fragment length diversity. The variables observed in this study were DNA quantitative analysis, the genotype, and alleles of the obtained genotype. The result shows the extracted DNA is of good quality because it has values >1.8 and
ISSN:2117-4458
2117-4458
DOI:10.1051/bioconf/20248800006