Thawed cryopreserved synovial mesenchymal stem cells show comparable effects to cultured cells in the inhibition of osteoarthritis progression in rats

Intra-articular injections of mesenchymal stem cells (MSCs) can inhibit the progression of osteoarthritis (OA). Previous reports have used cultured MSCs, but the ability to use thawed cryopreserved MSC stocks would be highly advantageous. Our purpose was to elucidate whether thawed cryopreserved MSC...

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Bibliographic Details
Published in:Scientific reports 2021-05, Vol.11 (1), p.9683-9683, Article 9683
Main Authors: Horiuchi, Kiyotaka, Ozeki, Nobutake, Endo, Kentaro, Mizuno, Mitsuru, Katano, Hisako, Akiyama, Masako, Tsuji, Kunikazu, Koga, Hideyuki, Sekiya, Ichiro
Format: Article
Language:English
Subjects:
631/532/2074
631/532/489
Arthritis
Cartilage
Cartilage diseases
Cryopreservation
Degeneration
Humanities and Social Sciences
Knee
Mesenchymal stem cells
multidisciplinary
Osteoarthritis
Science
Science (multidisciplinary)
Stem cell transplantation
Stem cells
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Summary:Intra-articular injections of mesenchymal stem cells (MSCs) can inhibit the progression of osteoarthritis (OA). Previous reports have used cultured MSCs, but the ability to use thawed cryopreserved MSC stocks would be highly advantageous. Our purpose was to elucidate whether thawed cryopreserved MSCs show comparable inhibitory effects on OA progression in rats to those obtained with cultured MSCs. Cultured rat synovial MSCs or thawed MSCs were compared for in vitro viability and properties. The inhibitory effect of thawed MSCs on OA progression was evaluated by injecting cryopreservation fluid and thawed MSCs in meniscectomized rats. Cartilage degeneration was assessed using gross finding and histological scores. Cultured MSCs were then injected into one knee and thawed MSCs into the contralateral knee of the same individual to compare their effects. Cultured MSCs and MSCs thawed after cryopreservation had comparable in vitro colony formation and chondrogenic potentials. In the rat OA model, the gross finding and histological scores were significantly lower in the thawed MSC group than in the cryopreservation fluid group at 8 weeks. Finally, cartilage degeneration did not differ significantly after injection of cultured and thawed MSCs. In conclusion, thawed MSCs showed comparable inhibitory effects on OA progression to cultured MSCs.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-89239-8