Anti-skin aging effects of mulberry fruit extracts: In vitro and in vivo evaluations of the anti-glycation and antioxidant activities

• Published in: Journal of functional foods 2024-01, Vol.112, p.105984, Article 105984
• Main Authors: Zhang, Lijun, Chen, Qirong, Chen, Zhang, He, Taoping, Yu, Mengjie, Zhang, Yehui, Nan, Haijun, Huang, Qingrong, Zhao, Tiantian
• Format: Article
• Language: English
• Subjects: AGEs; Anti-glycation; Anti-skin aging; Mulberry fruit extract; Oxidative stress
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2023.105984

Mulberry fruit extracts can improve D-galactose-induced aging by inhibiting in vitro and in vivo glycosylation and reducing oxidative stress levels in aging mice. [Display omitted] •The anti-glycation effect of mulberry fruit extracts (MFEs) was confirmed.•MFEs effectively alleviate MGO-induced oxidative stress in HaCaT cells.•Dried mulberry fruit extracts showed better anti-glycation effects in vitro and in vivo.•MFEs delay aging by alleviating D-galactose-induced oxidative stress. In this study, we investigated the bioactive compounds extracted from air-dried mulberry fruit (DMF), mulberry fruit juice (MFJ), and mulberry fruit pomace (MFP). We examined the effects of extracts from DMF, MFJ, and MFP on advanced glycation end-products (AGEs). The in vitro results showed significant inhibition of AGEs both in the fructose-BSA and MGO-BSA models. The results of compositional analysis showed that the phenolic content was partially positively correlated with their antioxidant capacity. Additionally, we investigated the potential of MFP extracts to improve MGO-induced aging-related deficits in HaCaT cells. Furthermore, the in vivo trial results showed that both DMF-W (with water extraction) and DMF-P (with Pectinex® XXL extraction) significantly increased the moisture, HA and HYP contents in the skin of mice, reduced the levels of AGEs, and improved the oxidative stress damage in aging mice. Additionally, DMF-P showed a better anti-skin aging effect.