Dengue: Rapid Diagnostic Testing in a Tertiary Care Setting in Butwal, Nepal

Dengue virus (DENV) is spread mostly by biting by the infected female mosquitoes of the species Aedes aegypti, that are commonly found in tropical and subtropical zones of globe. Some studies have shown that nearly fifty percent of the globe are susceptible to DENV. Most infections by DENV are asymp...

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Published in:Journal of pure & applied microbiology : an international research journal of microbiology 2023-03, Vol.17 (1), p.380-384
Main Authors: Khadka, Ram Bahadur, Neupane, Balram, Chaudhary, Gautam Prasad, Karki, Khimdhoj, Pokhrel, Arjun Prasad, Pant, Dhakaraj, Gupta, Santosh Kumar, Pandey, Jitendra, Gyawali, Rabin
Format: Article
Language:English
Subjects:
Analysis
Dengue
dengue fever
Dengue viruses
Diagnosis
Disease susceptibility
Educational software
immunoglobin g (igg)
immunoglobin m (igm)
Immunoglobulin G
Infection
Medical policy
Medical research
Medicine, Experimental
non-structural protein 1 (ns1)
rdts
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Summary:Dengue virus (DENV) is spread mostly by biting by the infected female mosquitoes of the species Aedes aegypti, that are commonly found in tropical and subtropical zones of globe. Some studies have shown that nearly fifty percent of the globe are susceptible to DENV. Most infections by DENV are asymptomatic. Clinical infections vary from mild fever to serious diseases including hemorrhagic fever and shock syndrome. The main objective of this study was to estimate DENV infection by rapid diagnostic (RDTs) kit methods in a tertiary care setting. This retrospective study was done at Crimson Hospital, Butwal, Nepal from June to November 2019. Serum samples of all dengue fever suspected patients were tested for DENV antigen as Dengue nonstructural protein 1(NS1) and DENV specific antibodies as IgM and IgG by using World Health Organization Good Manufacturing Practices (GMP) certified rapid solid phase immunochromatographic method. All laboratory test results were tabulated by using MS-Excel and analyzed by statistical package for the Social Sciences software version 20. Out of 821 samples tested, 518 were positive, including 322 (62.16%) reactive for dengue NS1 Protein, 121 (23.35%) for IgM, 62 (11.96%) for IgG and 13 (2.50%) were positive for both IgG and IgM antibodies. Our study showed high prevalence of dengue infection during monsoon to post-monsoon and the infection rate was higher in males (71.72%) than in females (53.36%). According to our findings, DENV infection is prevalent in Butwal, Nepal. Early diagnosis, better case management, faster public health response and effective health policy towards the control of mosquito vectors may reduce the dengue burden and must be implemented in Nepal.
ISSN:0973-7510
2581-690X
DOI:10.22207/JPAM.17.1.28