Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out

The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro tite...

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Bibliographic Details
Published in:Nanomaterials (Basel, Switzerland) Switzerland), 2021-04, Vol.11 (4), p.902
Main Authors: Lohse, Mirko, Thesen, Manuel W, Haase, Anja, Smolka, Martin, Iceta, Nerea Briz, Ayerdi Izquierdo, Ana, Ramos, Isbaal, Salado, Clarisa, Schleunitz, Arne
Format: Article
Language:English
Subjects:
Automation
Cytotoxicity
Drug screening
Fabrication
Manufacturing industry
Mastering
Microfluidics
nanoimprint lithography
Nanotechnology
neuronal cell assay
Neurons
Open channels
Plasma
Plates
Polymers
Polymethyl methacrylate
R2R UV-NIL
Silicon wafers
Ultraviolet radiation
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Summary:The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro titer plates per hour is presented. Deep insight into the evaluation of a suitable UV-curable material, mr-UVCur26SF is given, presenting cytotoxic evaluation, cell compatibility tests and finally a neuronal assay. The results indicate how the given pattern, in combination with the resist, paves the way to faster, cheaper, and more reliable drug screening.
ISSN:2079-4991
2079-4991
DOI:10.3390/nano11040902