Vitamin D Is Necessary for Murine Gastric Epithelial Homeostasis

Unlike other organs, the importance of VD in a normal stomach is unknown. This study focuses on understanding the physiological role of vitamin D in gastric epithelial homeostasis. C57BL/6J mice were divided into three groups that were either fed a standard diet and kept in normal light/dark cycles...

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Bibliographic Details
Published in:Biology (Basel, Switzerland) Switzerland), 2021-07, Vol.10 (8), p.705
Main Authors: Ali, Ifrah Ismail, Shah, Iltaf, Marzouk, Sayed, Karam, Sherif M., Al Menhali, Asma
Format: Article
Language:English
Subjects:
Antibodies
Apoptosis
Cell cycle
cell differentiation
Cell proliferation
Diet
Epithelial cells
Epithelium
Gastric cancer
gastric epithelial homeostasis
Gastrin
Gene expression
Homeostasis
Ligands
Nutrient deficiency
Parietal cells
Reagents
Stem cells
Stomach
Vitamin D
vitamin D receptor
Vitamin D receptors
vitamin D3
Vitamins
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Summary:Unlike other organs, the importance of VD in a normal stomach is unknown. This study focuses on understanding the physiological role of vitamin D in gastric epithelial homeostasis. C57BL/6J mice were divided into three groups that were either fed a standard diet and kept in normal light/dark cycles (SDL), fed a standard diet but kept in the dark (SDD) or fed a vitamin D-deficient diet and kept in the dark (VDD). After 3 months, sera were collected to measure vitamin D levels by LC-MS/MS, gastric tissues were collected for immunohistochemical and gene expression analyses and gastric contents were collected to measure acid levels. The VDD group showed a significant decrease in the acid-secreting parietal cell-specific genes Atp4a and Atp4b when compared with the controls. This reduction was associated with an increased expression of an antral gastrin hormone. VDD gastric tissues also showed a high proliferation rate compared with SDL and SDD using an anti-BrdU antibody. This study indicates the requirement for normal vitamin D levels for proper parietal cell functions.
ISSN:2079-7737
2079-7737
DOI:10.3390/biology10080705