Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing

Backcrossing is a powerful tool for plant breeding. The improved marker-assisted backcrossing intends to transfer targeted genes or quantitative trait loci (QTLs) of interest from a donor parent into a recurrent parent. In this study, a tobacco BC4F3 population was generated using Y3 and K326 as hyb...

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Published in:Czech Journal of Genetics and Plant Breeding 2021-01, Vol.57 (3), p.102-112
Main Authors: Tong, Zhijun, Jiang, Sanjie, He, Weiming, Chen, Xuejun, Yin, Lixin, Fang, Dunhuang, Hu, Yafei, Jiao, Fangchan, Zhang, Chi, Zeng, Jianmin, Wu, Xinfu, Zhao, Shancen, Jian, Jianbo, Xiao, Bingguang
Format: Article
Language:English
Subjects:
Biomarkers
crossing
Density
Gene mapping
Gene polymorphism
Gene sequencing
Genetic distance
Genomes
Next-generation sequencing
Nucleotides
Offspring
Plant breeding
Polymorphism
Population studies
Quantitative trait loci
single nucleotide polymorphism (snp)
Single-nucleotide polymorphism
Tobacco
Whole genome sequencing
whole genome sequencing (wgs)
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Summary:Backcrossing is a powerful tool for plant breeding. The improved marker-assisted backcrossing intends to transfer targeted genes or quantitative trait loci (QTLs) of interest from a donor parent into a recurrent parent. In this study, a tobacco BC4F3 population was generated using Y3 and K326 as hybrid parents and YF1-1 as F1 parents. High-throughput sequencing data of 381 pedigree populations were used to construct high-density genetic maps containing 24 142 high-quality single nucleotide polymorphism (SNP) markers with an average genetic distance of 0.59 cM. A genome module analysis was then performed for all the offspring. A total of forty-three candidate QTLs for six agronomics traits were identified. This study provides original biomarkers for tobacco breeding and offers clues for prospective backcrossing applications in other plants.
ISSN:1212-1975
1805-9325
DOI:10.17221/8/2021-CJGPB