Single amino acid in V2 encoded by TYLCV is responsible for its self-interaction, aggregates and pathogenicity

• Published in: Scientific reports 2018-02, Vol.8 (1), p.3561-11, Article 3561
• Main Authors: Zhao, Wenhao, Ji, Yinghua, Wu, Shuhua, Ma, Xiaofang, Li, Shuo, Sun, Feng, Cheng, Zhaobang, Zhou, Yijun, Fan, Yongjian
• Format: Article
• Language: English
• Subjects: 13/51; 631/208/2490/1472; 631/449/1659; 64; 82; 82/111; 82/80; 96; 96/35; 96/44; Amino Acid Sequence; Begomovirus - drug effects; Begomovirus - genetics; Begomovirus - pathogenicity; Cell Nucleus - virology; Cytoplasm; Cytoplasm - virology; Humanities and Social Sciences; Leupeptins - pharmacology; Localization; multidisciplinary; Mutagenesis, Site-Directed; Mutation; Nuclei; Pathogenicity; Plant Diseases - genetics; Plant Diseases - virology; Potexvirus - genetics; Proteasome 26S; Science; Science (multidisciplinary); Site-directed mutagenesis; Viral Proteins - genetics; Virus Diseases - genetics; Virus Diseases - virology; Yellow leaf
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-21446-2

The V2 protein encoded by Begomovirus is essential for virus infection and is involved in multiple functions, such as virus movement and suppression of the host defence response. In this study, we reported that V2 encoded by the Tomato yellow leaf curl virus (TYLCV), which is one of the most devastating tomato-infecting begomoviruses, could interact with itself and a S71A mutation of V2 (V2 S71A ) abolished its self-interaction. Fluorescence results showed that V2 localized primarily in the cytoplasm and around the nucleus. Site-directed mutagenesis V2 S71A had the similar subcellular localization, but V2 S71A formed fewer large aggregates in the cytoplasm compared to wild-type V2, whereas the level of aggregates came to a similar after treatment with MG132, which indicates that the S71A mutation might affect 26S proteasome-mediated degradation of V2 aggregates. Meanwhile, heterologous expression of V2 S71A from a Potato virus X vector induced mild symptoms compared to wild-type V2, delay of virus infection associated with mild symptoms was observed in plants inoculated with TYLCV-S71A, which indicates that the amino acid on position 71 is also involved in the pathogenicity of V2. To the best of our knowledge, this report is the first to state that the S71A mutation of V2 encoded by TYLCV affects the self-interaction, aggregate formation and pathogenicity of V2.