Influence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells

Because of the described hazards related to inhalation of manufactured nanoparticles, we investigated the lung toxicity of biodegradable poly (lactide-co-glycolide) (PLGA) nanoparticles displaying various surface properties on human bronchial Calu-3 cells. Positively and negatively charged as well a...

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Bibliographic Details
Published in:International journal of nanomedicine 2011-01, Vol.6 (default), p.2591-2605
Main Authors: Mura, Simona, Hillaireau, Herve, Nicolas, Julien, Le Droumaguet, Benjamin, Gueutin, Claire, Zanna, Sandrine, Tsapis, Nicolas, Fattal, Elias
Format: Article
Language:English
Subjects:
Bronchi - cytology
Bronchi - drug effects
Calu-3
Cell Line
Cell Survival - drug effects
Chitosan - chemistry
Humans
inflammation
Interleukin-6 - metabolism
Interleukin-8 - metabolism
Lactic Acid - chemistry
Lactic Acid - toxicity
Life Sciences
Microscopy, Confocal
nanoparticles
Nanoparticles - chemistry
Nanoparticles - toxicity
Original Research
PLGA
Poloxamer - chemistry
Polyglycolic Acid - chemistry
Polyglycolic Acid - toxicity
Polyvinyl Alcohol - chemistry
Rhodamines - chemistry
Static Electricity
Surface Properties
toxicity
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Summary:Because of the described hazards related to inhalation of manufactured nanoparticles, we investigated the lung toxicity of biodegradable poly (lactide-co-glycolide) (PLGA) nanoparticles displaying various surface properties on human bronchial Calu-3 cells. Positively and negatively charged as well as neutral nanoparticles were tailored by coating their surface with chitosan, Poloxamer, or poly (vinyl alcohol), respectively. Nanoparticles were characterized in terms of size, zeta potential, and surface chemical composition, confirming modifications provided by hydrophilic polymers. Although nanoparticle internalization by lung cells was clearly demonstrated, the cytotoxicity of the nanoparticles was very limited, with an absence of inflammatory response, regardless of the surface properties of the PLGA nanoparticles. These in vitro results highlight the safety of biodegradable PLGA nanoparticles in the bronchial epithelium and provide initial data on their potential effects and the risks associated with their use as nanomedicines.
ISSN:1178-2013
1176-9114
1178-2013
DOI:10.2147/IJN.S24552