In Vitro Evaluation of Some Endophytic Bacillus to Potentially Inhibit Grape and Grapevine Fungal Pathogens

• Published in: Plants (Basel) 2023-07, Vol.12 (13), p.2553
• Main Authors: Boiu-Sicuia, Oana-Alina, Toma, Radu Cristian, Diguță, Camelia Filofteia, Matei, Florentina, Cornea, Călina Petruța
• Format: Article
• Language: English
• Subjects: Agricultural industry; Antifungal activity; Bacillus; Bacillus spp; Bacteria; biocontrol; Biological control; Cellulase; Chitinase; Disease management; Endophytes; Environmental impact; Enzymes; Fungi; Fungicides; Genes; Grapes; grapevine; Grapevines; In vitro methods and tests; Lytic enzymes; molds; Pathogens; Phylogenetics; Plant diseases; Plant resistance; Polyamines; Ribosomal DNA; rRNA 16S; Solubilization; Spoilage; Strains (organisms); Sustainability management; Sustainable development; Temperature tolerance; trunk diseases; Viticulture; VOCs; Volatile organic compounds; Wineries & vineyards; Wines
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12132553

Romania has a long history of grapevine culturing and winemaking. However, like any agricultural sector, viticulture faces devastating biological threats. Fungi responsible for grapevine trunk diseases (GTDs) and grape spoilage lead to considerable yield losses and a decline in grapevine quality. In the actual context, many countries, including Romania, have reoriented their approaches to minimize chemical inputs, which have been proven to be toxic and to have negative impacts on the environment, and to replace them with sustainable biocontrol strategies for the wine-growing sector. Within biocontrol strategies, spp. is a well-known plant-protective bacteria with antifungal properties. Within this paper, six endophytic bacteria from various plant sources were studied. The bacterial strains were identified as , , and by sequencing their 16S rDNA region. Regardless of the in vitro test methods (using living bacterial cells, bacterial-cell-free supernatant (CFS), and volatile active compounds (VOCs)), strains revealed strong and broad antifungal activity against grape and grapevine fungal pathogens such as spp., , , , , spp., , , and . The functional antifungal genes encoding for difficidin, fengycin, iturins, macrolactin, and mycosubtilin were molecularly detected, which could support the proven antifungal activity of the endophytic strains. Lytic enzymes involved in fungal growth inhibition, such as chitinase, cellulase, and proteases, were also revealed to be produced by some of these bacterial strains. Various other in vitro tests, such as phosphate and phytate solubilization, phytohormone synthesis, the production of enzymes involved in the polyamine biosynthetic pathway, and pH as well as temperature tolerance tests were carried out to reveal the plant-beneficial potential of these bacterial strains. These results revealed that the strains, especially BAHs1, are the most suitable endophytes for grapevine biologic control, which could lead to the future development of sustainable management strategies.