Human Forebrain Organoid-Derived Extracellular Vesicle Labeling with Iron Oxides for In Vitro Magnetic Resonance Imaging

• Published in: Biomedicines 2022-11, Vol.10 (12), p.3060
• Main Authors: Liu, Chang, Helsper, Shannon, Marzano, Mark, Chen, Xingchi, Muok, Laureana, Esmonde, Colin, Zeng, Changchun, Sun, Li, Grant, Samuel C, Li, Yan
• Format: Article
• Language: English
• Subjects: Alzheimer's disease; Cameras; Drug delivery systems; Efficiency; extracellular vesicles; Flow cytometry; Forebrain; forebrain organoids; Foxg1 protein; Growth factors; human pluripotent stem cells; Inhibitory postsynaptic potentials; Intravenous administration; Iron oxides; Ischemia; Labeling; Magnetic resonance imaging; Nanoparticles; nanoscale iron oxides; Neurodegenerative diseases; Neuroimaging; Neurological diseases; Organoids; Physiological aspects; Physiology; Pluripotency; Polyethylene glycol; Prosencephalon; Size distribution; Software; Sonication; Stem cells; Thyroid transcription factor 1; Tubulin; α-Catenin
• ISSN: 2227-9059; 2227-9059
• DOI: 10.3390/biomedicines10123060

The significant roles of extracellular vesicles (EVs) as intracellular mediators, disease biomarkers, and therapeutic agents, make them a scientific hotspot. In particular, EVs secreted by human stem cells show significance in treating neurological disorders, such as Alzheimer’s disease and ischemic stroke. However, the clinical applications of EVs are limited due to their poor targeting capabilities and low therapeutic efficacies after intravenous administration. Superparamagnetic iron oxide (SPIO) nanoparticles are biocompatible and have been shown to improve the targeting ability of EVs. In particular, ultrasmall SPIO (USPIO,