A Simple Graphene Functionalized Electrochemical Aptasensor for the Sensitive and Selective Detection of Glycated Albumin

Glycated albumin (GA) has been previously introduced as a promising biomarker for glycemic monitoring in diabetes patients with thalassemia. In this study, a label-free graphene oxide (GO)-modified aptasensor was developed for the rapid detection of GA. The fabrication of the aptasensor was dependen...

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Bibliographic Details
Published in:Applied sciences 2021-11, Vol.11 (21), p.10315
Main Authors: Aye, Nang Noon Shean, Maraming, Pornsuda, Tavichakorntrakool, Ratree, Chaibunruang, Attawut, Boonsiri, Patcharee, Daduang, Sakda, Teawtrakul, Nattiya, Prasongdee, Prinya, Amornkitbamrung, Vittaya, Daduang, Jureerut
Format: Article
Language:English
Subjects:
Albumin
Albumins
Anemia
Aptamers
Biosensors
Carbon
Chromatography
Diabetes
Diabetes mellitus
electrochemical aptasensor
Electrochemistry
Electrodes
Fabrication
Glucose
glycated albumin
Graphene
graphene oxide
Hemoglobin
Nanomaterials
Patients
Proteins
Selectivity
Square waves
Thalassemia
Voltammetry
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Summary:Glycated albumin (GA) has been previously introduced as a promising biomarker for glycemic monitoring in diabetes patients with thalassemia. In this study, a label-free graphene oxide (GO)-modified aptasensor was developed for the rapid detection of GA. The fabrication of the aptasensor was dependent on the covalent interaction of the amine-functionalized GA-specific aptamer with the carboxylic groups of GO. Square wave voltammetry (SWV) analysis was carried out for the measurement of GA-aptamer binding to their specific proteins. The peak current changes before and after incubation with GA protein were directly proportional to the concentration. The developed aptasensor exhibited a broad linearity (1–10,000 µg mL−1), a low detection limit (LOD) of 0.031 µg mL−1, and high selectivity for GA detection. In addition, the aptasensor was successfully applied to detect GA in both spiked and clinical serum samples. The comparison of the developed method with a commercial assay validated the reliability of the aptasensor for clinical application. Therefore, the newly developed aptasensor is a promising tool for GA measurements in diabetic patients with underlying thalassemia.
ISSN:2076-3417
2076-3417
DOI:10.3390/app112110315