Enantiomeric Composition, Antioxidant Capacity and Anticholinesterase Activity of Essential Oil from Leaves of Chirimoya ( Annona cherimola Mill.)

• Published in: Plants (Basel) 2022-01, Vol.11 (3), p.367
• Main Authors: Valarezo, Eduardo, Ludeña, Jeannette, Echeverria-Coronel, Estefanía, Cartuche, Luis, Meneses, Miguel Angel, Calva, James, Morocho, Vladimir
• Format: Article
• Language: English
• Subjects: Acetylcholinesterase; Annona cherimola; antibacterial activity; Antiinfectives and antibacterials; antioxidant activity; Antioxidants; Bacteria; Bicyclogermacrene; Caryophyllene; Chemical composition; Cherimoya; Enantiomers; enantioselective analysis; essential oil; Essential oils; Flame ionization detectors; Flavor; Fruits; Gas chromatography; Germacrene; Gram-negative bacteria; Gram-positive bacteria; Hydrocarbons; Indigenous species; Ionization; Klebsiella; Minimum inhibitory concentration; Oils & fats; Parasitic diseases; Physical properties; Pinene; Qualitative analysis; Sabinene; Scavenging; Spectrophotometry; Tropical diseases
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants11030367

Mill. is a native species of Ecuador cultivated worldwide for the flavor and properties of its fruit. In this study, hydrodistillation was used to isolate essential oil (EO) of fresh leaves collected in Ecuadorian Sierra. The EO chemical composition was determined using a non-polar and a polar chromatographic column and enantiomeric distribution with an enantioselective column. The qualitative analysis was carried out by gas chromatography coupled to a mass spectrometer and quantitative analysis using gas chromatography equipped with a flame ionization detector. The antibacterial potency was assessed against seven Gram-negative bacteria and one Gram-positive bacterium. ABTS and DPPH assays were used to evaluate the radical scavenging properties of the EO. Spectrophotometric method was used to measure acetylcholinesterase inhibitory activity. GC-MS analysis allowed us to identify more than 99% of the EO chemical composition. Out of the fifty-three compounds identified, the main were germacrene D (28.77 ± 3.80%), sabinene (3, 9.05 ± 1.69%), β-pinene (4, 7.93 ± 0.685), (E)-caryophyllene (10.52 ± 1.64%) and bicyclogermacrene (11.12 ± 1.39%). Enantioselective analysis showed the existence of four pairs of enantiomers, the (-)-β-Pinene (1S, 5S) was found pure (100%). Chirimoya essential oil exhibited a strong antioxidant activity and a very strong anticholinesterase potential with an IC value of 41.51 ± 1.02 µg/mL. Additionally, EO presented a moderate activity against and with a MIC value of 500 μg/mL.