Expression profile of FGF23, alpha klotho, microRNA-126, and wnt signaling pathway gene polymorphism in patients with acute or chronic kidney diseases: relation to dialysis requirements

The connection between chronic kidney disease (CKD) and acute kidney injury (AKI) is becoming more widely acknowledged, as the two conditions probably exacerbate one other. The purpose of the present study was to evaluate the circulating levels of fibroblast growth factor 23 (FGF23), αKlotho, and mi...

Full description

Saved in:
Bibliographic Details
Published in:The Egyptian journal of internal medicine 2025-01, Vol.37 (1), p.5-17, Article 5
Main Authors: Hassan, Mohammed H., Saleem, Tahia H., Abdallah, Ahmed Alamir Mahmoud, Abdelhady, Marwa, Salama, Doha Abd-Elraheem, Elsaied, Abdelrahman A., Mohammed, Abdallah Elaiw, Hashim, Abdelkader Ahmed
Format: Article
Language:English
Subjects:
Acute kidney injury (AKI)
Cardiovascular disease
Chronic kidney disease (CKD)
Creatinine
Fibroblast growth factor 23
Gene expression
Genotype & phenotype
Hemodialysis
Internal Medicine
Kidney diseases
Medicine
Medicine & Public Health
MicroRNA-126
MicroRNAs
Patients
Plasma
Polymerase chain reaction (PCR)
Polymorphism
αKlotho
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The connection between chronic kidney disease (CKD) and acute kidney injury (AKI) is becoming more widely acknowledged, as the two conditions probably exacerbate one other. The purpose of the present study was to evaluate the circulating levels of fibroblast growth factor 23 (FGF23), αKlotho, and mircoRNA-126 (miR-126) and to explore the possible genetic role of single nucleotide polymorphisms (SNPs) in the klotho G-395A (rs1207568), C1818T (rs564481), and wnt signaling pathway AXIN-1 C > T (rs9921222) in AKI and CKD patients and their relation to progression of kidney disease and dialysis necessity. In this case–control study, there were 50 AKI patients, 100 CKD patients, and 50 healthy controls. ELISA assay kits were utilized to measure the plasma concentrations of FGF23 and α klotho, while reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) was used to measure the expression levels of miR-126. Using conventional PCR methods, rs1207568, rs564481 and rs9921222 SNPs were genetically analyzed. Patients with AKI and CKD had significantly higher median FGF23 levels than controls ( P  
ISSN:2090-9098
1110-7782
2090-9098
DOI:10.1186/s43162-024-00391-9