Iron Overload Is Associated With Oxidative Stress and Nutritional Immunity During Viral Infection in Fish

• Published in: Frontiers in immunology 2018-06, Vol.9, p.1296-1296
• Main Authors: Tarifeño-Saldivia, Estefanía, Aguilar, Andrea, Contreras, David, Mercado, Luis, Morales-Lange, Byron, Márquez, Katherine, Henríquez, Adolfo, Riquelme-Vidal, Camila, Boltana, Sebastian
• Format: Article
• Language: English
• Subjects: electronic paramagnetic resonance spectroscopy; Immunology; infectious pancreaticc necrosis virus; iron overload; nutritional immunity; oxidative stress; RNA-seq
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2018.01296

Iron is a trace element, essential to support life due to its inherent ability to exchange electrons with a variety of molecules. The use of iron as a cofactor in basic metabolic pathways is essential to both pathogenic microorganisms and their hosts. During evolution, the shared requirement of micro- and macro-organisms for this important nutrient has shaped the pathogen-host relationship. Infectious pancreatic necrosis virus (IPNv) affects salmonids constituting a sanitary problem for this industry as it has an important impact on post-smolt survival. While immune modulation induced by IPNv infection has been widely characterized on , viral impact on iron host metabolism has not yet been elucidated. In the present work, we evaluate short-term effect of IPNv on several infected tissues from . We observed that IPNv displayed high tropism to headkidney, which directly correlates with a rise in oxidative stress and antiviral responses. Transcriptional profiling on headkidney showed a massive modulation of gene expression, from which biological pathways involved with iron metabolism were remarkable. Our findings suggest that IPNv infection increase oxidative stress on headkidney as a consequence of iron overload induced by a massive upregulation of genes involved in iron metabolism.