Genome-Wide Identification of PR10 Family Members in Durum Wheat: Expression Profile and In Vitro Analyses of TdPR10.1 in Response to Various Stress Conditions

• Published in: Plants (Basel) 2024-11, Vol.13 (22), p.3128
• Main Authors: Khanfir, Emna, Zribi, Ikram, Dhouib, Hanen, Ghorbel, Mouna, Hamdi, Karama, Jrad, Olfa, Yacoubi, Inès, Brini, Faiçal
• Format: Article
• Language: English
• Subjects: abiotic and biotic stress; Abiotic stress; Amino acids; antifungal activity; Chromosomes; durum wheat; Evolutionary genetics; Flavonoids; Genes; Genetic aspects; genome-wide analysis; Genomes; Genomics; Gibberellins; Infections; LDH activity; Molecular weight; Pasta; Phylogenetics; Phylogeny; Plant growth; Plant species; PR10 family; Proteins; Regulatory sequences; Ribonuclease; Salinity; Triticum durum; Wheat
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13223128

The functional characterization of PR10 proteins has been extensively studied in many plant species. However, little is known about the role of TdPR10 in the response of durum wheat ( Desf.) to stress. In this study, we identified members of the PR10 family, which are divided into three major subfamilies based on phylogenetic analyses. The analysis revealed that tandem duplication was the primary driver of the expansion of the gene family. Additionally, gene structure and motif analyses showed that family genes were relatively conserved during evolution. We also identified several cis-regulatory elements in the promoter regions related not only to abiotic and biotic stress but also to phytohormonal responses. In response to abiotic stresses and phytohormones, several genes were highly expressed in the leaves and roots of durum wheat. Moreover, TdPR10.1 family members improve RNase activity, increase LDH protective activity under abiotic stress conditions, and ensure resistance to fungi in vitro. Collectively, these findings provide a basis for further functional studies of genes, which could be leveraged to enhance stress tolerance in durum wheat.