Saccharomyces cerevisiae DJ-1 paralogs maintain genome integrity through glycation repair of nucleic acids and proteins

• Published in: eLife 2023-08, Vol.12
• Main Authors: Susarla, Gautam, Kataria, Priyanka, Kundu, Amrita, D'Silva, Patrick
• Format: Article
• Language: English
• Subjects: Amino acids; Antibodies; Biochemistry and Chemical Biology; Carbonyl compounds; deglycation repair; DJ-1 family proteins; DNA damage; DNA glycation; Gene mutations; Genetic aspects; Genetic engineering; Genomes; Genomics; Genotoxicity; Glycolysis; Glycosylation; Homeostasis; Intermediates; Macromolecules; Mitochondria; mitochondrial dysfunction; mitochondrial glycation; Movement disorders; mRNA; Mutation; Neurodegenerative diseases; Neuroprotection; Neurotoxicity; Oxidation; PARK7 protein; Parkinson's disease; Physiological aspects; Physiology; Protein folding; Proteins; Proteomes; Pyruvaldehyde; RNA; Saccharomyces cerevisiae; Toxicity; Yeast; yeast Hsp31
• ISSN: 2050-084X; 2050-084X
• DOI: 10.7554/eLife.88875

Reactive carbonyl species (RCS) such as methylglyoxal and glyoxal are potent glycolytic intermediates that extensively damage cellular biomolecules leading to genetic aberration and protein misfolding. Hence, RCS levels are crucial indicators in the progression of various pathological diseases. Besides the glyoxalase system, emerging studies report highly conserved DJ-1 superfamily proteins as critical regulators of RCS. DJ-1 superfamily proteins, including the human DJ-1, a genetic determinant of Parkinson's disease, possess diverse physiological functions paramount for combating multiple stressors. Although retains four DJ-1 orthologs (Hsp31, Hsp32, Hsp33, and Hsp34), their physiological relevance and collective requirement remain obscure. Here, we report for the first time that the yeast DJ-1 orthologs function as novel enzymes involved in the preferential scavenge of glyoxal and methylglyoxal, toxic metabolites, and genotoxic agents. Their collective loss stimulates chronic glycation of the proteome, and nucleic acids, inducing spectrum of genetic mutations and reduced mRNA translational efficiency. Furthermore, the Hsp31 paralogs efficiently repair severely glycated macromolecules derived from carbonyl modifications. Also, their absence elevates DNA damage response, making them vulnerable to various genotoxins. Interestingly, yeast DJ-1 orthologs preserve functional mitochondrial content, maintain ATP levels, and redistribute into mitochondria to alleviate the glycation damage of macromolecules. Together, our study uncovers a novel glycation repair pathway in and a possible neuroprotective mechanism of how hDJ-1 confers mitochondrial health during glycation toxicity.