Flexible Multi-Beam Light-Sheet Fluorescence Microscope for Live Imaging Without Striping Artifacts

The development of light-sheet fluorescence microscopy (LSFM) has greatly expanded the experimental capabilities in many biological and biomedical research fields, enabling for example live studies of murine and zebrafish neural activity or of cell growth and division. The key feature of the method...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in neuroanatomy 2019-02, Vol.13, p.7-7
Main Authors: Sancataldo, Giuseppe, Gavryusev, Vladislav, de Vito, Giuseppe, Turrini, Lapo, Locatelli, Massimiliano, Fornetto, Chiara, Tiso, Natascia, Vanzi, Francesco, Silvestri, Ludovico, Pavone, Francesco Saverio
Format: Article
Language:English
Subjects:
acousto optic deflector
brain imaging
fast volumetric imaging
Illumination
Lasers
Light
light-sheet fluorescence microscopy
Microscopy
Neuroscience
Optics
Sectioning
striping artifacts
zebrafish
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The development of light-sheet fluorescence microscopy (LSFM) has greatly expanded the experimental capabilities in many biological and biomedical research fields, enabling for example live studies of murine and zebrafish neural activity or of cell growth and division. The key feature of the method is the selective illumination of a sample single plane, providing an intrinsic optical sectioning and allowing direct 2D image recording. On the other hand, this excitation scheme is more affected by absorption or scattering artifacts in comparison to point scanning methods, leading to un-even illumination. We present here an easily implementable method, based on acousto-optical deflectors (AOD), to overcome this obstacle. We report the advantages provided by flexible and fast AODs in generating simultaneous angled multiple beams from a single laser beam and in fast light sheet pivoting and we demonstrate the suppression of illumination artifacts.
ISSN:1662-5129
1662-5129
DOI:10.3389/fnana.2019.00007