A Response Surface Methodology (RSM) Approach for Optimizing the Attenuation of Human IgE-Reactivity to β-Lactoglobulin (β-Lg) by Hydrostatic High Pressure Processing

The response surface methodology (RSM) and central composite design (CCD) technique were used to optimize the three key process parameters (i.e., pressure, temperature and holding time) of the high-hydrostatic-pressure (HHP) processing either standalone or combined with moderate thermal processing t...

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Bibliographic Details
Published in:Foods 2021-07, Vol.10 (8), p.1741
Main Authors: Sun, Xin, Vivien Chua, Jialing, Le, Quynh Anh, Trujillo, Francisco J., Oh, Mi-Hwa, Campbell, Dianne E., Mehr, Sam, Lee, Nanju Alice
Format: Article
Language:English
Subjects:
Allergens
Attenuation
Binding
Design optimization
Dimerization
Dimers
Enzymes
Food
Food science
high hydrostatic pressure processing
High pressure
human IgE-binding
Immunoglobulin E
Immunoglobulin G
Laboratories
Lactalbumin
Lactoglobulin
Milk
Molecular structure
Molecular weight
Peptides
Pressure
Process parameters
Proteins
Quadratic equations
rabbit IgG binding
Response surface methodology
response surface models
Sodium
Statistical analysis
α-lactalbumin
β-Lactoglobulin
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Summary:The response surface methodology (RSM) and central composite design (CCD) technique were used to optimize the three key process parameters (i.e., pressure, temperature and holding time) of the high-hydrostatic-pressure (HHP) processing either standalone or combined with moderate thermal processing to modulate molecular structures of β-lactoglobulin (β-Lg) and α-lactalbumin (α-La) with reduced human IgE-reactivity. The RSM model derived for HHP-induced molecular changes of β-Lg determined immunochemically showed that temperature (temp), pressure (p2) and the interaction between temperature and time (t) had statistically significant effects (p < 0.05). The optimal condition defined as minimum (β-Lg specific) IgG-binding derived from the model was 505 MPa at 56 °C with a holding time of 102 min (R2 of 0.81 and p-value of 0.01). The validation carried at the optimal condition and its surrounding region showed that the model to be underestimating the β-Lg structure modification. The molecular change of β-Lg was directly correlated with HHP-induced dimerization in this study, which followed a quadratic equation. The β-Lg dimers also resulted in the undetectable human IgE-binding.
ISSN:2304-8158
2304-8158
DOI:10.3390/foods10081741