Magnesium Sulfate Mitigates the Progression of Monocrotaline Pulmonary Hypertension in Rats

• Published in: International journal of molecular sciences 2019-09, Vol.20 (18), p.4622
• Main Authors: Chang, Chao-Yuan, Shih, Hung-Jen, Huang, I-Tao, Tsai, Pei-Shan, Chen, Kung-Yen, Huang, Chun-Jen
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Apoptosis - drug effects; Calcium Channel Blockers - therapeutic use; Disease Progression; Hypertension; Hypertension, Pulmonary - chemically induced; Hypertension, Pulmonary - drug therapy; Hypertension, Pulmonary - pathology; Inflammasomes; Inflammation; Injuries; Laboratories; Lipid peroxidation; Lipids; Magnesium sulfate; Magnesium Sulfate - therapeutic use; Male; Mitochondria; Monocrotaline; oxidation; Proteins; Pulmonary arteries; Pulmonary Artery - drug effects; Pulmonary Artery - pathology; Pulmonary hypertension; Rats; Rats, Sprague-Dawley; right heart failure; Transmission electron microscopy; Tumor necrosis factor-TNF; Veins & arteries
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20184622

We investigated whether magnesium sulfate (MgSO ) mitigated pulmonary hypertension progression in rats. Pulmonary hypertension was induced by a single intraperitoneal injection of monocrotaline (60 mg/kg). MgSO (100 mg/kg) was intraperitoneally administered daily for 3 weeks, from the seventh day after monocrotaline injection. Adult male rats were randomized into monocrotaline (MCT) or monocrotaline plus MgSO (MM) groups ( = 15 per group); control groups were maintained simultaneously. For analysis, surviving rats were euthanized on the 28 day after receiving monocrotaline. The survival rate was higher in the MM group than in the MCT group (100% versus 73.3%, = 0.043). Levels of pulmonary artery wall thickening, α-smooth muscle actin upregulation, right ventricular systolic pressure increase, and right ventricular hypertrophy were lower in the MM group than in the MCT group (all < 0.05). Levels of lipid peroxidation, mitochondrial injury, inflammasomes and cytokine upregulation, and apoptosis in the lungs and right ventricle were lower in the MM group than in the MCT group (all < 0.05). Notably, the mitigation effects of MgSO on pulmonary artery wall thickening and right ventricular hypertrophy were counteracted by exogenous calcium chloride. In conclusion, MgSO mitigates pulmonary hypertension progression, possibly by antagonizing calcium.