Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application

Strigolactones (SLs) are intriguing phytohormones that not only regulate plant development and architecture but also interact with other organisms in the rhizosphere as root parasitic plants ( Striga, Orobanche , and Phelipanche ) and arbuscular mycorrhizal fungi. Starting with a pioneering work in...

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Published in:Frontiers in plant science 2022-06, Vol.13, p.887347-887347
Main Authors: de Saint Germain, Alexandre, Clavé, Guillaume, Schouveiler, Paul, Pillot, Jean-Paul, Singh, Abhay-Veer, Chevalier, Arnaud, Daignan Fornier, Suzanne, Guillory, Ambre, Bonhomme, Sandrine, Rameau, Catherine, Boyer, François-Didier
Format: Article
Language:English
Subjects:
Arabidopsis thaliana
Biochemistry
Biochemistry, Molecular Biology
Botanics
Chemical Sciences
Life Sciences
Organic chemistry
pea
Physcomitrium patens
Plant Science
profluorescent probes
strigolactone
Vegetal Biology
α/β-hydrolases
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Summary:Strigolactones (SLs) are intriguing phytohormones that not only regulate plant development and architecture but also interact with other organisms in the rhizosphere as root parasitic plants ( Striga, Orobanche , and Phelipanche ) and arbuscular mycorrhizal fungi. Starting with a pioneering work in 2003 for the isolation and identification of the SL receptor in parasitic weeds, fluorescence labeling of analogs has proven a major strategy to gain knowledge in SL perception and signaling. Here, we present novel chemical tools for understanding the SL perception based on the enzymatic properties of SL receptors. We designed different profluorescent SL Guillaume Clavé (GC) probes and performed structure-activity relationship studies on pea, Arabidopsis thaliana , and Physcomitrium (formerly Physcomitrella ) patens . The binding of the GC probes to PsD14/RMS3, AtD14, and OsD14 proteins was tested. We demonstrated that coumarin-based profluorescent probes were highly bioactive and well-adapted to dissect the enzymatic properties of SL receptors in pea and a resorufin profluorescent probe in moss, contrary to the commercially available fluorescein profluorescent probe, Yoshimulactone Green (YLG). These probes offer novel opportunities for the studies of SL in various plants.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2022.887347