Comparative Expression Profiling Reveals Molecular Markers Involved in the Progression of Cutaneous Melanoma towards Metastasis

Cutaneous melanoma is one of the most aggressive types of cancer and often proves fatal in metastatic stages. Few treatment options are available, and its global incidence is quickly increasing. In order to gain an improved understanding of the molecular features regarding melanoma progression, we h...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-03, Vol.24 (7), p.6565
Main Authors: Lazăr, Andreea D, Dinescu, Sorina, Sleiman, Lea, Dumitru, Adrian V, Costache, Mariana, Costache, Marieta
Format: Article
Language:English
Subjects:
Angiogenesis
Biomarkers
Brain cancer
brain metastasis
Care and treatment
Cell cycle
Comparative analysis
Computer software industry
Confocal microscopy
cutaneous melanoma
EGFR
Extracellular matrix
Gene expression
Gene Expression Profiling - methods
Genes
Humans
IL1B
Immunohistochemistry
Instrument industry
Kinases
lymph node metastasis
Lymph nodes
Melanoma
Melanoma - pathology
Melanoma, Cutaneous Malignant
Metastases
Metastasis
MicroRNA
MicroRNAs
MicroRNAs - genetics
Microscopy
miRNA
MMP2
Motility
Neoplasm Metastasis
Protein expression
Proteins
Scientific equipment and supplies industry
Skin cancer
Skin Neoplasms - pathology
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Summary:Cutaneous melanoma is one of the most aggressive types of cancer and often proves fatal in metastatic stages. Few treatment options are available, and its global incidence is quickly increasing. In order to gain an improved understanding of the molecular features regarding melanoma progression, we have compared gene and small non-coding RNA expression profiles from cell lines derived from primary melanoma (MelJuSo), lymph node metastasis (MNT-1) and brain metastasis (VMM1), representing distinct stages of malignant progression. Our preliminary results highlighted the aberrant regulation of molecular markers involved in several processes that aid melanoma progression and metastasis development, including extracellular matrix remodeling, migratory potential and angiogenesis. Moreover, bioinformatic analysis revealed potential targets of the microRNAs of interest. Confocal microscopy and immunohistochemistry analysis were used for validation at the protein level. Exploring the molecular landscape of melanoma may contribute to the achievement of future efficient targeted therapy, as well as better prevention, diagnosis and clinical management.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24076565