A New Long Noncoding RNA ALB Regulates Autophagy by Enhancing the Transformation of LC3BI to LC3BII during Human Lens Development

Autophagy is essential in lens organelle degradation. This study aimed to seek potential autophagy-associated long noncoding RNAs (lncRNAs) and their relative mechanisms in human lens development using the “fried egg” lentoid body (LB) generation system. The expression pattern of LC3B in differentia...

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Bibliographic Details
Published in:Molecular therapy. Nucleic acids 2017-12, Vol.9 (C), p.207-217
Main Authors: Fu, Qiuli, Qin, Zhenwei, Zhang, Lifang, Lyu, Danni, Tang, Qiaomei, Yin, Houfa, Chen, Zhijian, Yao, Ke
Format: Article
Language:English
Subjects:
Aging
Autophagy
Cataracts
Embryo cells
Epithelial cells
Gene expression
Homeostasis
induced pluripotent stem cells
Inhibitory postsynaptic potentials
lens
lentoid bodies
long noncoding RNA
Phagocytosis
Pluripotency
Ribonucleic acid
RNA
Stem cell transplantation
Stem cells
Studies
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Summary:Autophagy is essential in lens organelle degradation. This study aimed to seek potential autophagy-associated long noncoding RNAs (lncRNAs) and their relative mechanisms in human lens development using the “fried egg” lentoid body (LB) generation system. The expression pattern of LC3B in differentiating LBs was similar to that in developing a mouse lens in vivo. Among the massive lncRNAs expressed with a significant difference between induced pluripotent stem cells (iPSCs) and LBs, lncRNA affecting LC3B (ALB), which was predicted to have a co-relationship with the autophagy marker LC3B, was highly expressed in differentiating lens fibers in LBs. This result was consistent with its high expression in human embryonic lenses compared to those in embryonic stem cells (ESCs). Furthermore, lncRNA ALB knockdown resulted in the downregulation of LC3BII at the protein level, therefore inhibiting the autophagy process in human lens epithelial cells (HLECs). Our results identify lncRNA ALB, a potential autophagy regulator in organelle degradation during human lens development, highlighting the importance of lncRNAs in lens development. [Display omitted]
ISSN:2162-2531
2162-2531
DOI:10.1016/j.omtn.2017.09.011