The maternal reduced uteroplacental perfusion model of preeclampsia induces sexually dimorphic metabolic responses in rat offspring

• Published in: Biology of sex differences 2022-09, Vol.13 (1), p.1-48, Article 48
• Main Authors: Hassanzadeh-Taheri, Mohammadmehdi, Mohammadifard, Mahtab, Erfanian, Zahra, Hosseini, Mehran
• Format: Article
• Language: English
• Subjects: Abdomen; Animal models; Blood pressure; Body mass; Bupivacaine; Carotid arteries; Catheters; Children; Coronary vessels; Diabetes; Diabetes mellitus; Drinking water; Fetuses; Ghrelin; Glucagon; Glucose; Glucose tolerance; Glucose tolerance tests; Growth disorders; Hypertension; Immunohistochemistry; Immunoreactivity; Insulin; Insulin resistance; Investigations; Juveniles; Laboratory animals; Males; Metabolic disorders; Metabolic dysfunction; Metabolic response; Obesity; Offspring; Ovaries; Pancreas; Perfusion; Povidone; Pre-eclampsia; Preeclampsia; Pregnancy; Proteins; Rodents; Sexual dimorphism; Type 2 diabetes; Urine; Veins & arteries; Young adults
• ISSN: 2042-6410; 2042-6410
• DOI: 10.1186/s13293-022-00458-8

Background Offspring born to preeclamptic mothers are prone to obesity, diabetes and hypertension in later life, but still, studies investigating the underlying mechanism are limited. Here, we aimed to investigate the impact of the reduced uteroplacental perfusion (RUPP) rat preeclampsia model on offspring metabolic outcomes. Methods Timed pregnant Wistar rats underwent RUPP or sham surgeries on day 14 of gestation. Glucometabolic parameters were evaluated on postnatal days (PND), 14 (childhood), and 60 (young adult). In addition, intraperitoneal glucose tolerance test (IPGTT), homeostatic model assessment of insulin resistance (HOMA-IR), immunohistochemical staining for insulin in pancreatic islets, arterial blood pressure and 24-h urine protein (24hUP) excretion were performed at PND60. Results Male, but not female, young adult rats (PND60) of RUPP dams exhibited an impaired IPGTT, decreased circulatory insulin and weakened pancreatic insulin immunoreactivity. Compared to the male offspring of the sham group, the body mass of male RUPP offspring significantly caught up after PND42, but it was not sex-specific. RUPP pups also exhibited upregulations in glucagon (only males) and ghrelin (both sexes with a more significant increase in males) during PND14-PND60. However, in sham offspring (both sexes), glucagon levels were downregulated and ghrelin levels unchanged during PND14-PND60. The blood pressure, HOMA-IR and 24hUP values did not alter in RUPP pups. Conclusions The overall results suggest that maternal RUPP has negative and sex-specific impacts on insulin, glucagon and ghrelin regulations in offspring and that, as young adults, male RUPP rats may be more prone to develop obesity and diabetes. Keywords: Preeclampsia, Offspring, Growth disorders, Metabolic dysfunction, Diabetes, Ghrelin