Circular RNA FOXO3 Suppresses Bladder Cancer Progression and Metastasis by Regulating MiR-9-5p/TGFBR2

Increasing evidence indicates that the dysregulation of circular RNAs (circRNAs) plays important roles in tumor progressions. In this study, we first analyzed circ-FOXO3 level in bladder cancers (BCs), and then BC cell lines were transfected with circ-FOXO3 expression vector, and cell proliferation,...

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Bibliographic Details
Published in:Cancer management and research 2020-01, Vol.12, p.5049-5056
Main Authors: Li, Yongxiang, Qiao, Liang, Zang, Yuanwei, Ni, Wenjun, Xu, Zhonghua
Format: Article
Language:English
Subjects:
Analysis
Bladder cancer
Cancer metastasis
circ-foxo3
Development and progression
Luciferase
MicroRNA
mir-9-5p
Original Research
tgfbr2
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Summary:Increasing evidence indicates that the dysregulation of circular RNAs (circRNAs) plays important roles in tumor progressions. In this study, we first analyzed circ-FOXO3 level in bladder cancers (BCs), and then BC cell lines were transfected with circ-FOXO3 expression vector, and cell proliferation, migration, and invasion abilities were analyzed. We also used bioinformatics tools to predict potential-binding miRNAs for circ-FOXO3, and luciferase reporter assay was used for the verification of binding miRNAs. For the further study, we analyzed potential downstream-binding mRNA for miRNA, and cell proliferation, migration and invasion abilities of it were also studied. We found that circ-FOXO3 was significantly down-regulated in bladder cancer (BC) tissues compared to normal bladder tissues. We also found that circ-FOXO3 overexpression inhibited cell proliferation, migration and invasion in BC cell lines. Moreover, we demonstrated that TGFBR2 was regulated by circ-FOXO3 through sponging miR-9-5p, the knockdown of TGFBR2 or the overexpression of miR-9-5p all related to the increased BC cell proliferation, migration, and invasion. In summary, our data showed that circ-FOXO3 was significantly down-regulated in bladder cancers. circ-FOXO3 overexpression inhibits BC cell progression and metastasis. Furthermore, circ-FOXO3 regulates TGFBR2 expression through sponging miR-9-5p in BC cell lines.
ISSN:1179-1322
1179-1322
DOI:10.2147/CMAR.S253412