Patellar instability-induced bone loss in the femoral trochlea is associated with the activation of the JAK1/STAT3 signaling pathway in growing mice

Patellar instability (PI) at an early age is believed closely correlated with bone loss in the development of the femoral trochlea and can cause trochlear dysplasia. However, the molecular mechanism of PI-induced bone loss has not been established. The Janus kinase (JAK)/signal transducers and activ...

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Published in:Journal of orthopaedic surgery and research 2023-07, Vol.18 (1), p.526-526, Article 526
Main Authors: Ma, Chen, Kou, Wenguan, Cui, Zhaoxia, Liu, Wenfeng, Liu, Changli, Wang, Shengjie, Wang, Fei
Format: Article
Language:English
Subjects:
Analysis
Animals
Antibodies
Bone Diseases, Metabolic
Bone loss
Bone mass
Computed tomography
Cytokines
Dysplasia
Femur
Goats
Immunohistochemistry
JAK1/STAT3
Janus kinase
Joint Instability
Kinases
Laboratory animals
Male
Mice
Mice, Inbred C57BL
Molecular modelling
Orthopedics
Osteoclasts
Osteoprotegerin
Patellar instability
Patellofemoral Joint
Proteins
Sample size
Signal transduction
Stat3 protein
Statistical analysis
Subchondral bone
Surgery
Surgical techniques
TRANCE protein
Transcription factors
Trochlear dysplasia
X-Ray Microtomography
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Summary:Patellar instability (PI) at an early age is believed closely correlated with bone loss in the development of the femoral trochlea and can cause trochlear dysplasia. However, the molecular mechanism of PI-induced bone loss has not been established. The Janus kinase (JAK)/signal transducers and activators of transcription (STAT) signaling pathway plays an important role in bone development by regulating the expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa B ligand (RANKL). The aim of this study was to explore the association of JAK1/STAT3 signaling to PI-induced subchondral bone loss in the femoral trochlea. Four-week-old male C57BL/6 mice were randomly divided into two groups (n = 50/group). Mice in the experimental group underwent surgery to induce PI. Distal femurs were collected 2 and 4 weeks after surgery (n = 25 knees/each time point, each group). Microcomputed tomography and histological observations were performed to investigate the morphology of the femoral trochlea and changes in bone mass. qPCR, western blot, and immunohistochemistry analyses were performed to evaluate the expression of JAK1, STAT3, RANKL, and OPG in subchondral bone. A t test was performed for the statistical analysis; a P value 
ISSN:1749-799X
1749-799X
DOI:10.1186/s13018-023-04019-6