Transcriptome Analysis of Psacothea hilaris: De Novo Assembly and Antimicrobial Peptide Prediction

• Published in: Insects (Basel, Switzerland) Switzerland), 2020-10, Vol.11 (10), p.676
• Main Authors: Lee, Joon Ha, Chung, Hoyong, Shin, Yong Pyo, Kim, In-Woo, Natarajan, Sathishkumar, Veerappan, Karpagam, Seo, Minchul, Park, Junhyung, Hwang, Jae Sam
• Format: Article
• Language: English
• Subjects: Amino acids; Antibiotics; Antifungal activity; Antimicrobial activity; Antimicrobial agents; antimicrobial peptide; Antimicrobial peptides; Antiparasitic agents; Apoptosis; Bacteria; Beetles; Cell death; E coli; Fungi; Fungicides; Gene expression; Hemolymph; hemolysis; Immunization; Insects; knottin; Microorganisms; Multidrug resistance; Pathogens; Peptides; Permeability; Physiochemistry; Psacothea hilaris; psacotheasin; Ribonucleic acid; RNA; Sodium; transcriptome; Transcriptomes; Yeasts
• ISSN: 2075-4450; 2075-4450
• DOI: 10.3390/insects11100676

Antimicrobial peptides (AMPs) are the frontline innate defense system evolutionarily preserved in insects to combat invading pathogens. These AMPs could serve as an alternative to classical antibiotics to overcome the burden of treating multidrug resistant bacteria. Psacotheasin, a knottin type AMP was isolated from Psacothea hilaris and shown to exhibit antimicrobial activity, especially against fungi through apoptosis mediated cell death. In this study, we aimed to identify novel probable AMPs from Psacothea hilaris, the yellow spotted longicorn beetle. The beetle was immunized with the two bacterial strains (E. coli and S. aureus), and the yeast strain C. albicans. After immunization, total RNA was isolated and sequenced in Illumina platform. Then, beetle transcriptome was de novo assembled and searched for putative AMPs with the known physiochemical features of the AMPs. A selection of AMP candidates were synthesized and tested for antimicrobial activity. Four peptides showed stronger activity against E. coli than the control AMP, melittin while one peptide showed similar activity against S. aureus. Moreover, four peptides and two peptides showed antifungal activity stronger than and similar to melittin, respectively. Collectively one peptide showed both antibacterial and antifungal activity superior to melittin; thus, it provides a potent antimicrobial peptide. All the peptides showed no hemolysis in all the tested concentrations. These results suggest that in silico mining of insects’ transcriptome could be a promising tool to obtain and optimize novel AMPs for human needs.