Tissue microarray analyses of the essential DNA repair factors ATM, DNA-PKcs and Ku80 in head and neck squamous cell carcinoma

• Published in: Radiation oncology (London, England) England), 2024-10, Vol.19 (1), p.150-11, Article 150
• Main Authors: Zech, Henrike Barbara, von Bargen, Clara, Oetting, Agnes, Möckelmann, Nikolaus, Möller-Koop, Christina, Witt, Melanie, Struve, Nina, Petersen, Cordula, Betz, Christian, Rothkamm, Kai, Münscher, Adrian, Clauditz, Till Sebastian, Rieckmann, Thorsten
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Analysis; Ataxia Telangiectasia Mutated Proteins - metabolism; ATM; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; Cancer; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; Carcinoma, Squamous Cell - radiotherapy; Carcinoma, Squamous Cell - virology; DNA; DNA damage; DNA microarrays; DNA Repair; DNA-Activated Protein Kinase - metabolism; DNA-PKcs; Female; Genetic aspects; Genetic research; Head and Neck Neoplasms - metabolism; Head and Neck Neoplasms - pathology; Head and Neck Neoplasms - radiotherapy; Head and Neck Neoplasms - virology; HNSCC; Humans; Ku Autoantigen - metabolism; Ku80; Male; Middle Aged; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Papillomavirus infections; Prognosis; Prognostic marker; Squamous cell carcinoma; Squamous Cell Carcinoma of Head and Neck - pathology; Squamous Cell Carcinoma of Head and Neck - radiotherapy; Squamous Cell Carcinoma of Head and Neck - virology; Tissue Array Analysis; Tissue microarray
• ISSN: 1748-717X; 1748-717X
• DOI: 10.1186/s13014-024-02541-3

Head and neck squamous cell carcinoma (HNSCC) negative for Human Papillomavirus (HPV) has remained a difficult to treat entity, whereas tumors positive for HPV are characterized by radiosensitivity and favorable patient outcome. On the cellular level, radiosensitivity is largely governed by the tumor cells` ability to repair radiation-induced DNA double-strand breaks (DSBs), but no biomarker is established that could guide clinical decision making. Therefore, we tested the impact of the expression levels of ATM, the central kinase of the DNA damage response as well as DNA-PKcs and Ku80, two major factors in the main DSB repair pathway non-homologous end joining (NHEJ). A tissue microarray of a single center HNSCC cohort was stained for ATM, DNA-PKcs and Ku80 and the expression scored based on staining intensity and the percentages of tumor cells stained. Scores were correlated with clinicopathological parameters and survival. Samples from 427 HNSCC patients yielded interpretable stainings and were scored following an established algorithm. The majority of tumors showed strong expression of both NHEJ factors, whereas the expression of ATM varied more. The expression scores of ATM and DNA-PKcs were not associated with patient survival. For HPV-negative HNSCC, the minority of tumors without strong Ku80 expression trended towards superior survival when treatment included radiotherapy. Focusing stronger on staining intensity to define the subgroup with lowest and therefore potentially insufficient expression levels in the HPV-negative subgroup, we observed significantly better overall survival for patients treated with radiotherapy but not with surgery alone. Our data suggest that HPV-negative HNSCC with particularly low Ku80 expression represent a highly radiosensitive subpopulation. Confirmation in independent cohorts is required.