Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization

A "green" solvent-free industrial process (patent pending) is here described for a grape seed extract (GSE) preparation (Ecovitis™) obtained from selected seeds of Veneto region wineries, in the northeast of Italy, by water and selective tangential flow filtration at different porosity. Si...

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Bibliographic Details
Published in:Antioxidants 2021-03, Vol.10 (3), p.418
Main Authors: Morazzoni, Paolo, Vanzani, Paola, Santinello, Sandro, Gucciardi, Antonina, Zennaro, Lucio, Miotto, Giovanni, Ursini, Fulvio
Format: Article
Language:English
Subjects:
Acids
Calibration
Catechin
catechins
Chromophores
ESI-Q-TOF mass spectrometry
gel permeation chromatography
grapeseed extract
Mass spectroscopy
Plant extracts
Polyphenols
Porosity
Proanthocyanidins
Quality control
Seeds
Species
Stereoisomers
Wineries
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Summary:A "green" solvent-free industrial process (patent pending) is here described for a grape seed extract (GSE) preparation (Ecovitis™) obtained from selected seeds of Veneto region wineries, in the northeast of Italy, by water and selective tangential flow filtration at different porosity. Since a comprehensive, non-ambiguous characterization of GSE is still a difficult task, we resorted to using an integrated combination of gel permeation chromatography (GPC) and electrospray ionization high resolution mass spectrometry (ESI-HRMS). By calibration of retention time and spectroscopic quantification of catechin as chromophore, we succeeded in quantifying GPC polymers up to traces at = 30. The MS analysis carried out by the ESI-HRMS method by direct-infusion allows the detection of more than 70 species, at different polymerization and galloylation, up to = 13. This sensitivity took advantage of the nanoscale shotgun approach, although paying the limit of missed separation of stereoisomers. GPC and MS approaches were remarkably well cross-validated by overlapping results. This simple integrated analytical approach has been used for quality control of the production of Ecovitis™. The emerging feature of Ecovitis™ vs. a popular benchmark in the market, produced by a different technology, is the much lower content of species at low and the corresponding increase of species at high .
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox10030418