Upregulation of microRNA-451 increases cisplatin sensitivity of non-small cell lung cancer cell line (A549)

• Published in: Journal of experimental & clinical cancer research 2011-02, Vol.30 (1), p.20-20, Article 20
• Main Authors: Bian, Hai-Bo, Pan, Xuan, Yang, Jin-Song, Wang, Zhao-Xia, De, Wei
• Format: Article
• Language: English
• Subjects: Animals; Antineoplastic Agents - therapeutic use; Apoptosis; Apoptosis - genetics; Cancer; Cancer therapies; Carcinoma, Non-Small-Cell Lung - drug therapy; Carcinoma, Non-Small-Cell Lung - genetics; Care and treatment; Cell Line, Tumor; Chemotherapy; Cisplatin; Cisplatin - therapeutic use; Dosage and administration; Drug Resistance, Neoplasm - genetics; Female; Formazans; Gene Expression Regulation, Neoplastic; Genetic Vectors; Health aspects; Humans; Lung cancer; Lung cancer, Non-small cell; Lung Neoplasms - drug therapy; Lung Neoplasms - genetics; Membranes; Mice; Mice, Inbred BALB C; MicroRNA; MicroRNAs - genetics; Patient outcomes; Physiological aspects; Polymerase chain reaction; Proteins; Science; Signal Transduction - genetics; Tetrazolium Salts; Transfection; Up-Regulation - genetics
• ISSN: 1756-9966; 0392-9078; 1756-9966
• DOI: 10.1186/1756-9966-30-20

Recently, miR-451 as a tumor suppressor has been reported in other studies. However, whether miR-451 can affect the sensitivity of non-small cell lung cancer (NSCLC) cells to cisplatin (DDP) remains unclear. The aim of this study is to evaluate the roles of miR-451 in the sensitivity of NSCLC cells to DDP. Quantitative RT-PCR assay was performed to detect the expression of miR-451 in 10 pairs of NSCLC and noncancerous tissue samples. pcDNA-GW/EmGFP-miR-451 was stably transfected into NSCLC cell line (A549). Then, the effects of miR-451 upregulation on growth, colony formation and apoptosis of A549 cells were investigated. Finally, the effects of miR-451 upregulation on in vitro and in vivo sensitivity of A549 cells of DDP were also determined. The level of miR-451 expression in NSCLC tissues was significantly higher than that in corresponding noncancerous tissues. Ectopic overexpression of miR-451 could significantly inhibit growth and induce apoptosis of A549 cells. Moreover, ectopic overexpression of miR-451 could sensitize A549 cells to DDP possibly by increasing DDP-induced apoptosis which might be associated with the inactivation of Akt signaling pathway. This study demonstrated for the first time that combination of DDP application with miR-451 upregulation might be a potential strategy for the treatment of human NSCLC.