Methylated RNA Immunoprecipitation Sequencing Reveals the m6A Landscape in Oral Squamous Cell Carcinoma

N6-methyladenosine (m6A) is the most common epigenetic modification existing in eukaryocyte transcripts. However, genes related to m6A modification in oral squamous cell carcinoma (OSCC) are still unclear. Here, methylated RNA immunoprecipitation sequencing (MeRIP-Seq) was performed to map the m6A l...

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Bibliographic Details
Published in:Journal of immunology research 2022-07, Vol.2022, p.1-13
Main Authors: Wang, Xi, Wu, Jie, Zhang, Leyu, Zhao, Wei, Deng, Jiayin
Format: Article
Language:English
Subjects:
3' Untranslated regions
Cancer
Epigenetics
Genes
Genomes
Hedgehog protein
Immunology
Immunoprecipitation
MicroRNAs
N6-methyladenosine
NF-κB protein
Non-coding RNA
Ontology
Oral cancer
Oral carcinoma
Oral squamous cell carcinoma
Ribonucleic acid
RNA
Signal transduction
Software packages
Squamous cell carcinoma
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Summary:N6-methyladenosine (m6A) is the most common epigenetic modification existing in eukaryocyte transcripts. However, genes related to m6A modification in oral squamous cell carcinoma (OSCC) are still unclear. Here, methylated RNA immunoprecipitation sequencing (MeRIP-Seq) was performed to map the m6A landscape in OSCC and corresponding controls. The m6A peaks are always distributed in the junction of the 3′-untranslated regions (3′-UTRs) and the coding sequences (CDS) of mRNAs, as well as the entire genome of long noncoding RNA (lncRNA). Furthermore, enrichment analysis showed that differentially methylated genes were significantly enriched in NF-kappa B signaling pathway, Hedgehog signaling pathway, etc. In summary, our findings reveal the landscape of m6A modification on mRNAs and lncRNAs in OSCC, which may provide key clues for the precision-targeted therapy of OSCC.
ISSN:2314-8861
2314-7156
DOI:10.1155/2022/7277583