Fermentative production and optimization of mevastatin in submerged fermentation using Aspergillus terreus

The main objective of the study is to enhance the mevastatin production using Plackett–Burman (PB) and central composite design (CCD) by Aspergillus terreus in submerged fermentation (SmF). Eight nutrients were chosen for a PB design with 12 experimental runs. A maximum mevastatin production of 170....

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Bibliographic Details
Published in:Biotechnology reports (Amsterdam, Netherlands) Netherlands), 2015-06, Vol.6 (C), p.124-128
Main Authors: Syed, Mahin Basha, Rajasimman, M.
Format: Article
Language:English
Subjects:
Aspergillus terreus
Mevastatin
PB design
Response surface methodology
Submerged fermentation
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Summary:The main objective of the study is to enhance the mevastatin production using Plackett–Burman (PB) and central composite design (CCD) by Aspergillus terreus in submerged fermentation (SmF). Eight nutrients were chosen for a PB design with 12 experimental runs. A maximum mevastatin production of 170.4mgL−1 was obtained in PB design. Response surface methodology (RSM) is a sequential procedure with an initial objective to lead the experimenter rapidly and efficiently along a path of improvement toward the general vicinity of the optimum. The individual and interactive effects of these variables were studied by conducting the fermentation run at randomly selected and different levels of all five factors. Experiments were conducted to optimize the medium constituents like glycerol, CuCl2·2H2O, FeSO4·7H2O, KH2PO4 and MgSO4·7H2O. At the optimum condition, a maximum mevastatin production of 701mgL−1 was obtained.
ISSN:2215-017X
2215-017X
DOI:10.1016/j.btre.2015.04.002