Synergistic Effect between Amoxicillin and Zinc Oxide Nanoparticles Reduced by Oak Gall Extract against Helicobacter pylori

Helicobacter pylori (H. pylori) is a global health threat, and the World Health Organization has included H. pylori among 12 bacterial species that require high priority future strategies for the development of new antibiotics due mainly to its high rates of resistance. Metallic nanoparticles are kn...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2022-07, Vol.27 (14), p.4559
Main Authors: Attia, Hany G., Albarqi, Hassan A., Said, Ismail G., Alqahtani, Omaish, Raey, Mohamed A. EI
Format: Article
Language:English
Subjects:
Acids
Amoxicillin
Antibiotics
Antiinfectives and antibacterials
Antimicrobial agents
Bacteria
Biocompatibility
checkerboard assay
Evaluation
Gallic acid
Global health
Health risks
Helicobacter pylori
LC-MS/MS
Metabolites
Metal oxides
Nanoparticles
Oak galls
Oxidative stress
Particle size
Penicillin
Phenols
Public health
Stomach
Synergism
Synergistic effect
Ulcers
Zinc oxide
zinc oxide nanoparticles
Zinc oxides
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Summary:Helicobacter pylori (H. pylori) is a global health threat, and the World Health Organization has included H. pylori among 12 bacterial species that require high priority future strategies for the development of new antibiotics due mainly to its high rates of resistance. Metallic nanoparticles are known for their antimicrobial properties. The FDA (Food and Drug Administration) has approved zinc oxide nanoparticles (ZnONPs) as biocompatible antimicrobials. Green synthesis of ZnONPs was performed based on Oak galls extract (OGE) and was characterized by UV, IR, DLS, TEM, and SEM measurements. In addition, LC-MS/MS was used for the identification of OGE constituents. A checkerboard assay was used to evaluate the activity of synthesized Qi-ZnONPs and OGE against H. pylori, and their synergistic effects with amoxicillin were evaluated. LC-MS/MS analyses identified 20 compounds as major gallic acid conjugates. The ZnONPs had average particle sizes of 5.5 nm (DLS) and 7.99 nm (TEM). Both OGE and Qi-ZnONPs exhibited moderate activity against H. pylori. Amoxicillin and Qi-ZnONPs combinations (1:2 and 1:4 amoxicillin:/Qi-ZnONPs) significantly decreased the MIC90 by two-fold and four-fold, respectively, and FIC values for the combinations were more significant than with OGE alone. OGE is rich in phenolics. The synergism between Qi-ZnONPs and amoxicillin can provide an alternative safe agent of low cost to combat H. Pylori infections.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules27144559