The Role of Rho-GTPases and actin polymerization during Macrophage Tunneling Nanotube Biogenesis

• Published in: Scientific reports 2017-08, Vol.7 (1), p.8547-16, Article 8547
• Main Authors: Hanna, Samer J., McCoy-Simandle, Kessler, Miskolci, Veronika, Guo, Peng, Cammer, Michael, Hodgson, Louis, Cox, Dianne
• Format: Article
• Language: English
• Subjects: 13; 13/1; 13/106; 13/109; 13/89; 14; 14/33; 14/34; 14/35; 14/63; 631/250; 631/80/128/1276; Actin; Actins - metabolism; Animals; Biosensors; Biosynthesis; cdc42 GTP-Binding Protein - metabolism; Cdc42 protein; Cell Communication; Cell Line; Cell Surface Extensions - metabolism; Fluorescence resonance energy transfer; Guanine nucleotide-binding protein; Guanosine triphosphatases; Humanities and Social Sciences; Humans; Localization; Macrophages; Macrophages - cytology; Macrophages - metabolism; Mice; multidisciplinary; Nanotechnology; Nanotubes; Organelles; Polymerization; rac1 GTP-Binding Protein - metabolism; Rac1 protein; rho GTP-Binding Proteins - metabolism; Science; Science (multidisciplinary); Signal Transduction; Studies; Time-Lapse Imaging - methods; Wiskott-Aldrich syndrome; Wiskott-Aldrich Syndrome Protein - genetics; Wiskott-Aldrich Syndrome Protein - metabolism; Wiskott-Aldrich Syndrome Protein Family - metabolism
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-08950-7

Macrophage interactions with other cells, either locally or at distances, are imperative in both normal and pathological conditions. While soluble means of communication can transmit signals between different cells, it does not account for all long distance macrophage interactions. Recently described tunneling nanotubes (TNTs) are membranous channels that connect cells together and allow for transfer of signals, vesicles, and organelles. However, very little is known about the mechanism by which these structures are formed. Here we investigated the signaling pathways involved in TNT formation by macrophages using multiple imaging techniques including super-resolution microscopy (3D-SIM) and live-cell imaging including the use of FRET-based Rho GTPase biosensors. We found that formation of TNTs required the activity and differential localization of Cdc42 and Rac1. The downstream Rho GTPase effectors mediating actin polymerization through Arp2/3 nucleation, Wiskott-Aldrich syndrome protein (WASP) and WASP family verprolin-homologous 2 (WAVE2) proteins are also important, and both pathways act together during TNT biogenesis. Finally, TNT function as measured by transfer of cellular material between cells was reduced following depletion of a single factor demonstrating the importance of these factors in TNTs. Given that the characterization of TNT formation is still unclear in the field; this study provides new insights and would enhance the understanding of TNT formation towards investigating new markers.