How Have Leukocyte In Vitro Chemotaxis Assays Shaped Our Ideas about Macrophage Migration?

Macrophage chemotaxis is crucial during both onset and resolution of inflammation and unique among all leukocytes. Macrophages are able to switch between amoeboid and mesenchymal migration to optimise their migration through 3D environments. This subtle migration phenotype has been underappreciated...

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Bibliographic Details
Published in:Biology (Basel, Switzerland) Switzerland), 2020-12, Vol.9 (12), p.439
Main Authors: Rumianek, Agata N, Greaves, David R
Format: Article
Language:English
Subjects:
Bacteria
Cell adhesion & migration
Chemotaxis
Extracellular matrix
Fibroblasts
in vitro assays
Inflammation
leukocyte
Leukocyte migration
macrophage
Macrophages
Mesenchyme
Metastasis
Phenotypes
Review
transwell
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Summary:Macrophage chemotaxis is crucial during both onset and resolution of inflammation and unique among all leukocytes. Macrophages are able to switch between amoeboid and mesenchymal migration to optimise their migration through 3D environments. This subtle migration phenotype has been underappreciated in the literature, with macrophages often being grouped and discussed together with other leukocytes, possibly due to the limitations of current chemotaxis assays. Transwell assays were originally designed in the 1960s but despite their long-known limitations, they are still one of the most popular methods of studying macrophage migration. This review aims to critically evaluate transwell assays, and other popular chemotaxis assays, comparing their advantages and limitations in macrophage migration studies.
ISSN:2079-7737
2079-7737
DOI:10.3390/biology9120439