An Engineered Laccase from Fomitiporia mediterranea Accelerates Lignocellulose Degradation

Laccases from white-rot fungi catalyze lignin depolymerization, a critical first step to upgrading lignin to valuable biodiesel fuels and chemicals. In this study, a wildtype laccase from the basidiomycete (Fom_lac) and a variant engineered to have a carbohydrate-binding module (Fom_CBM) were studie...

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Bibliographic Details
Published in:Biomolecules (Basel, Switzerland) Switzerland), 2024-03, Vol.14 (3), p.324
Main Authors: Pham, Le Thanh Mai, Deng, Kai, Choudhary, Hemant, Northen, Trent R, Singer, Steven W, Adams, Paul D, Simmons, Blake A, Sale, Kenneth L
Format: Article
Language:English
Subjects:
Basidiomycota - metabolism
Biodegradation
Biodiesel fuels
Biofuels
Carbohydrates
Cellulases
Cellulose
Depolymerization
Enzymes
Ethers
Fomitiporia mediterranea
Fomitiporia mediterranea
Fungi
Komagataella pastoris expression
Laccase
Laccase - metabolism
Lignin
Lignin - chemistry
Lignocellulose
Mass spectrometry
Mass spectroscopy
Microorganisms
nanostructure-initiator mass spectrometry (NIMS)
Oxidation
Phenolic compounds
Raw materials
Scientific imaging
Sodium
Sugars
White rot
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Summary:Laccases from white-rot fungi catalyze lignin depolymerization, a critical first step to upgrading lignin to valuable biodiesel fuels and chemicals. In this study, a wildtype laccase from the basidiomycete (Fom_lac) and a variant engineered to have a carbohydrate-binding module (Fom_CBM) were studied for their ability to catalyze cleavage of β-O-4' ether and C-C bonds in phenolic and non-phenolic lignin dimers using a nanostructure-initiator mass spectrometry-based assay. Fom_lac and Fom_CBM catalyze β-O-4' ether and C-C bond breaking, with higher activity under acidic conditions (pH < 6). The potential of Fom_lac and Fom_CBM to enhance saccharification yields from untreated and ionic liquid pretreated pine was also investigated. Adding Fom_CBM to mixtures of cellulases and hemicellulases improved sugar yields by 140% on untreated pine and 32% on cholinium lysinate pretreated pine when compared to the inclusion of Fom_lac to the same mixtures. Adding either Fom_lac or Fom_CBM to mixtures of cellulases and hemicellulases effectively accelerates enzymatic hydrolysis, demonstrating its potential applications for lignocellulose valorization. We postulate that additional increases in sugar yields for the Fom_CBM enzyme mixtures were due to Fom_CBM being brought more proximal to lignin through binding to either cellulose or lignin itself.
ISSN:2218-273X
2218-273X
DOI:10.3390/biom14030324