Irrigation with Magnetized Water Alleviates the Harmful Effect of Saline–Alkaline Stress on Rice Seedlings

• Published in: International journal of molecular sciences 2022-09, Vol.23 (17), p.10048
• Main Authors: Ma, Changkun, Li, Qian, Song, Zhaoxin, Su, Lijun, Tao, Wanghai, Zhou, Beibei, Wang, Quanjiu
• Format: Article
• Language: English
• Subjects: Abiotic stress; Alkaline soils; Chlorophyll; Crop production; Drought; Gene expression; Homeostasis; Investigations; Irrigation; magnetized water; Metabolism; Molecular modelling; Nitrates; Nitrogen; nitrogen absorption; Photosynthesis; Physiology; Plant growth; Rice; rice (Oryza sativa); Saline soils; saline–alkaline stress; Seedlings; Soil stresses; Water treatment
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms231710048

Saline–alkaline stress suppresses rice growth and threatens crop production. Despite substantial research on rice’s tolerance to saline–alkaline stress, fewer studies have examined the impact of magnetic water treatments on saline–alkaline-stressed rice plants. We explored the physiological and molecular mechanisms involved in saline–alkaline stress tolerance enhancement via irrigation with magnetized water using Nipponbare. The growth of Nipponbare plants was inhibited by saline–alkaline stress, but this inhibition was alleviated by irrigating the plants with magnetized water, as evidenced by greater plant height, biomass, chlorophyll content, photosynthetic rates, and root system in plants irrigated with magnetized water compared to those irrigated with non-magnetized water. Plants that were irrigated with magnetized water were able to acquire more total nitrogen. In addition, we proved that rice seedlings irrigated with magnetized water had a greater root NO3−-nitrogen concentration and root NH4+-nitrogen concentration than plants irrigated with non-magnetized water. These findings suggest that treatment with magnetized water could increase nitrogen uptake. To test this hypothesis, we analyzed the expression levels of genes involved in nitrogen acquisition. The expression levels of OsNRT1;1, OsNRT1;2, OsNRT2;1, OsAMT1;2, OsAMT2;1, OsAMT2;2, OsAMT2;3, OsAMT3;1, OsAMT3;2, and OsAMT3;3 were higher in plants exposed to magnetized water medium compared to those exposed to non-magnetized water media. We further demonstrated that treatment with magnetized water increases available nitrogen, NO3−-nitrogen content, and NH4+-nitrogen content in soil under saline–alkaline stress. Our results revealed that the increased resistance of rice seedlings to saline–alkaline stress may be attributable to a very effective nitrogen acquisition system enhanced by magnetized water.