Testing the Neuroprotective Properties of PCSO-524 ® Using a Neuronal Cell Cycle Suppression Assay

Cell cycle reentry is a unified mechanism shared by several neurodegenerative diseases, including Alzheimer's disease (AD) and Ataxia Telangiectasia (A-T). This phenotype is often related to neuroinflammation in the central nervous system. To mimic brain inflammation in vitro, we adopted the pr...

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Bibliographic Details
Published in:Marine drugs 2019-01, Vol.17 (2), p.79
Main Authors: Zhu, Beika, Zhang, Yang, Herrup, Karl
Format: Article
Language:English
Subjects:
Alzheimer's disease
Alzheimers disease
Animal models
Apoptosis
Ataxia
Ataxia telangiectasia
Brain
Cell cycle
Cell differentiation
Cell division
Cells
Central nervous system
Chemokines
Cytokines
Diseases
Fatty acids
Inflammation
Morphology
Neurodegeneration
Neurodegenerative diseases
neuroinflammation
neuronal cell cycle event
Neurons
Neuroprotection
PCSO-524
Phenotypes
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Summary:Cell cycle reentry is a unified mechanism shared by several neurodegenerative diseases, including Alzheimer's disease (AD) and Ataxia Telangiectasia (A-T). This phenotype is often related to neuroinflammation in the central nervous system. To mimic brain inflammation in vitro, we adopted the previously established method of using conditioned medium collected from activated THP-1 cells and applied it to both differentiated HT22 cells and primary neurons. Unscheduled cell cycle events were observed in both systems, indicating the potential of this approach as an in vitro model of neurodegenerative disease. We used this assay to measure the neuroprotective effects of New Zealand green-lipped mussel extract, PCSO-524 , to protect post-mitotic cells from cell cycle reentry. We found that, both in vitro and in an animal model, PCSO-524 displayed promising neuroprotective effects, and thus has potential to postpone or prevent the onset of neurodegenerative disease.
ISSN:1660-3397
1660-3397
DOI:10.3390/md17020079