Integrated SMRT and Illumina Sequencing Provide New Insights into Crocin Biosynthesis of Gardenia jasminoides

Crocins are valuable bioactive components of gardenia fruit, and their biosynthesis and accumulation have attracted widespread interest. Studies have investigated the biosynthesis and accumulation of crocin based on Illumina sequencing, but there is a lack of reports based on full-length transcripto...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-06, Vol.23 (11), p.6321
Main Authors: Shen, Tengfei, Zheng, Yongjie, Liu, Qian, Chen, Caihui, Huang, Lili, Deng, Shaoyong, Xu, Meng, Yang, Chunxia
Format: Article
Language:English
Subjects:
Accumulation
Biosynthesis
Carotenoids - analysis
Chromatography, High Pressure Liquid - methods
crocin biosynthesis
crocin content
DNA sequencing
Enzymes
Fruit - chemistry
Fruit - genetics
Fruits
Gardenia - chemistry
Gardenia - genetics
Gardenia jasminoides
Gene expression
Genes
Genomes
High performance liquid chromatography
High-Throughput Nucleotide Sequencing
Iridoids - analysis
Isoforms
MicroRNAs
Proteins
RNA-seq
Roles
SMRT sequencing
Software
Transcription factors
Transcriptomes
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Summary:Crocins are valuable bioactive components of gardenia fruit, and their biosynthesis and accumulation have attracted widespread interest. Studies have investigated the biosynthesis and accumulation of crocin based on Illumina sequencing, but there is a lack of reports based on full-length transcriptome sequencing. Utilising SMRT sequencing and high-performance liquid chromatography (HPLC), we explored crocin biosynthesis and accumulation in the fruit of . HPLC analysis showed that crocins specifically exist in fruit and that the content of crocins increases gradually during fruit development. SMRT sequencing generated 46,715 high-quality full-length isoforms, including 5230 novel isoforms that are not present in the genome. Furthermore, a total of 46 genes and 91 lncRNAs were involved in the biosynthesis and accumulation of crocin. The qRT-PCR indicated that genes involved in crocin biosynthesis reached a peak in the NOV stage. These findings contributed to our understanding of crocin biosynthesis and accumulation.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23116321