Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization

Staphylococcus aureus (Gram-positive) and Pseudomonas aeruginosa (Gram-negative) bacteria represent major infectious threats in the hospital environment due to their wide distribution, opportunistic behavior, and increasing antibiotic resistance. This study reports on the deposition of polyvinylpyrr...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2021-06, Vol.26 (12), p.3634
Main Authors: Grumezescu, Valentina, Negut, Irina, Cristescu, Rodica, Grumezescu, Alexandru Mihai, Holban, Alina Maria, Iordache, Florin, Chifiriuc, Mariana Carmen, Narayan, Roger J., Chrisey, Douglas B.
Format: Article
Language:English
Subjects:
Antibiotic resistance
Antibiotics
Antimicrobial agents
Bacteria
Bacterial infections
Biocompatibility
Biofilms
ceftriaxone
cefuroxime
Coatings
Colonization
Composite materials
Drug resistance
Endothelial cells
Evaporation
Flavonoids
Fourier transforms
Gram-negative bacteria
Incubation
Infectious diseases
Infrared spectroscopy
isoflavonoid
Lasers
luteone
Medical equipment
Microorganisms
Microscopy
Nanomaterials
Phytochemicals
Polyvinylpyrrolidone
Pseudomonas aeruginosa
Pulsed lasers
Scanning electron microscopy
Severe acute respiratory syndrome coronavirus 2
Spectrum analysis
Staphylococcus infections
Thin films
wighteone
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Summary:Staphylococcus aureus (Gram-positive) and Pseudomonas aeruginosa (Gram-negative) bacteria represent major infectious threats in the hospital environment due to their wide distribution, opportunistic behavior, and increasing antibiotic resistance. This study reports on the deposition of polyvinylpyrrolidone/antibiotic/isoflavonoid thin films by the matrix-assisted pulsed laser evaporation (MAPLE) method as anti-adhesion barrier coatings, on biomedical surfaces for improved resistance to microbial colonization. The thin films were characterized by Fourier transform infrared spectroscopy, infrared microscopy, and scanning electron microscopy. In vitro biological assay tests were performed to evaluate the influence of the thin films on the development of biofilms formed by Gram-positive and Gram-negative bacterial strains. In vitro biocompatibility tests were assessed on human endothelial cells examined for up to five days of incubation, via qualitative and quantitative methods. The results of this study revealed that the laser-fabricated coatings are biocompatible and resistant to microbial colonization and biofilm formation, making them successful candidates for biomedical devices and contact surfaces that would otherwise be amenable to contact transmission.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26123634