Increased Aquaporin-7 Expression Is Associated with Changes in Rat Brown Adipose Tissue Whitening in Obesity: Impact of Cold Exposure and Bariatric Surgery

• Published in: International journal of molecular sciences 2023-02, Vol.24 (4), p.3412
• Main Authors: Frühbeck, Gema, Méndez-Giménez, Leire, Becerril, Sara, Ramírez, Beatriz, Hernández-Pardos, Ana Wenting, Cienfuegos, Javier A, Valentí, Víctor, Moncada, Rafael, Catalán, Victoria, Gómez-Ambrosi, Javier, da Silva, Inês V, Soveral, Graça, Rodríguez, Amaia
• Format: Article
• Language: English
• Subjects: adipocyte hypertrophy; Adipocytes; Adipose tissue; Adipose tissue (brown); Adipose Tissue, Brown - metabolism; Adipose tissues; Animals; aquaglyceroporin; Aquaporins; Aquaporins - metabolism; Bariatric Surgery; Bioaccumulation; Body fat; Cell differentiation; Cold; Endothelial cells; Endothelial Cells - metabolism; Enzymes; Exposure; Fatty acids; Gastrectomy; Gastrointestinal surgery; Gene expression; Ghrelin; Glycerin; Glycerol; Glycerol - metabolism; Hypertrophy; Instrument industry; Insulin; Insulin resistance; Leptin; Lipids; lipogenesis; Male; Metabolism; Metabolites; Morphology; Obesity; Obesity - metabolism; peroxisome proliferator-activated receptor γ; Protein expression; Proteins; Rats; Rats, Wistar; RNA; sleeve gastrectomy; Steatosis; Surgery; Thermogenesis; Transcription factors; Triglycerides; Type 2 diabetes; Weight control
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24043412

Glycerol is a key metabolite for lipid accumulation in insulin-sensitive tissues. We examined the role of aquaporin-7 (AQP7), the main glycerol channel in adipocytes, in the improvement of brown adipose tissue (BAT) whitening, a process whereby brown adipocytes differentiate into white-like unilocular cells, after cold exposure or bariatric surgery in male Wistar rats with diet-induced obesity (DIO) ( = 229). DIO promoted BAT whitening, evidenced by increased BAT hypertrophy, steatosis and upregulation of the lipogenic factors , and . AQP7 was detected in BAT capillary endothelial cells and brown adipocytes, and its expression was upregulated by DIO. Interestingly, AQP7 gene and protein expressions were downregulated after cold exposure (4 °C) for 1 week or one month after sleeve gastrectomy in parallel to the improvement of BAT whitening. Moreover, mRNA expression was positively associated with transcripts of the lipogenic factors , and and regulated by lipogenic (ghrelin) and lipolytic (isoproterenol and leptin) signals. Together, the upregulation of AQP7 in DIO might contribute to glycerol influx used for triacylglycerol synthesis in brown adipocytes, and hence, BAT whitening. This process is reversible by cold exposure and bariatric surgery, thereby suggesting the potential of targeting BAT AQP7 as an anti-obesity therapy.