The effect of Maras powder and smoking on the microRNA deregulation of oral mucosa

This study aimed to investigate the effects of Maras powder (a type of smokeless tobacco obtained from Nicotiana rustica Linn and mixed with the ashes of wood, especially from oak, walnut or grapevine) on the microRNA (miRNA) deregulation of oral mucosa, and it compares these effects with those of s...

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Bibliographic Details
Published in:Journal of applied oral science 2020-01, Vol.28, p.e20190382-e20190382
Main Authors: Taş, Betül, Güre, Ali Osmay
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Analysis of Variance
Cross-Sectional Studies
Dentistry
DENTISTRY, ORAL SURGERY & MEDICINE
Down-Regulation
Female
Gene Expression
Humans
Male
MicroRNAs
MicroRNAs - analysis
MicroRNAs - drug effects
Middle Aged
Mouth mucosa
Mouth Mucosa - drug effects
Mouth Neoplasms - genetics
Original
Powders
Real-Time Polymerase Chain Reaction
Risk Factors
Smokeless tobacco
Smoking
Time Factors
Tobacco, Smokeless - adverse effects
Young Adult
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Summary:This study aimed to investigate the effects of Maras powder (a type of smokeless tobacco obtained from Nicotiana rustica Linn and mixed with the ashes of wood, especially from oak, walnut or grapevine) on the microRNA (miRNA) deregulation of oral mucosa, and it compares these effects with those of smoking. Oral mucosal samples were collected from 74 patients, consisting of 16 nonusers, 26 smokers, and 32 Maras powder users. Genes associated with oral cancer were selected and 90 microRNAs targeting these genes were identified. MicroRNA were isolated and purified using the microRNA isolation kit. MicroRNA were expressed using Fluidigm RT-PCR. A positive correlation between the duration of Maras powder use with miR-31 expression levels, and a negative correlation between the Maras powder chewing time and miR-372 expression levels was found. In addition, there is a negative correlation between the amount of Maras powder consumed and expression levels of miR-375, miR-378a, miR-145, and miR-10b; moreover, another negative correlation is observed between the number of cigarettes consumed and the expression levels of miR-23a, miR-23b, miR-203a, miR-200b, and miR-375. However, miR-200b and miR-92a levels were downregulated significantly more in Maras powder users when compared with smokers and nonusers (p
ISSN:1678-7757
1678-7765
1678-7765
DOI:10.1590/1678-7757-2019-0382