Synthetic protein interactions reveal a functional map of the cell

To understand the function of eukaryotic cells, it is critical to understand the role of protein-protein interactions and protein localization. Currently, we do not know the importance of global protein localization nor do we understand to what extent the cell is permissive for new protein associati...

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Bibliographic Details
Published in:eLife 2016-04, Vol.5, p.e13053-e13053
Main Authors: Berry, Lisa K, Ólafsson, Guðjón, Ledesma-Fernández, Elena, Thorpe, Peter H
Format: Article
Language:English
Subjects:
Cell Biology
Cluster analysis
Defects
GBP
Genes
GFP
Homeostasis
Kinases
Laboratories
Localization
Microscopy
nanobody
Observations
Protein Binding
Protein interaction
Protein Interaction Maps
Protein Transport
Protein-protein interactions
Proteins
Saccharomyces cerevisiae - physiology
Saccharomyces cerevisiae Proteins - metabolism
Software
Tools and Resources
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Summary:To understand the function of eukaryotic cells, it is critical to understand the role of protein-protein interactions and protein localization. Currently, we do not know the importance of global protein localization nor do we understand to what extent the cell is permissive for new protein associations - a key requirement for the evolution of new protein functions. To answer this question, we fused every protein in the yeast Saccharomyces cerevisiae with a partner from each of the major cellular compartments and quantitatively assessed the effects upon growth. This analysis reveals that cells have a remarkable and unanticipated tolerance for forced protein associations, even if these associations lead to a proportion of the protein moving compartments within the cell. Furthermore, the interactions that do perturb growth provide a functional map of spatial protein regulation, identifying key regulatory complexes for the normal homeostasis of eukaryotic cells.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.13053