Placental Villous Explant Culture 2.0: Flow Culture Allows Studies Closer to the In Vivo Situation

During pregnancy, freely floating placental villi are adapted to fluid shear stress due to placental perfusion with maternal plasma and blood. In vitro culture of placental villous explants is widely performed under static conditions, hoping the conditions may represent the in utero environment. How...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-07, Vol.22 (14), p.7464
Main Authors: Kupper, Nadja, Pritz, Elisabeth, Siwetz, Monika, Guettler, Jacqueline, Huppertz, Berthold
Format: Article
Language:English
Subjects:
Apoptosis
Blood flow
Cell Culture Techniques
Chorionic gonadotropin
Chorionic Gonadotropin - metabolism
Chorionic Villi - growth & development
Chorionic Villi - metabolism
Cytokeratin
Cytoskeleton
explant culture under flow
Explants
Female
Fetuses
flow culture system
Flow Cytometry
Flow velocity
Fluid flow
Gonadotropins
Humans
Immunohistochemistry
In vivo methods and tests
L-Lactate dehydrogenase
L-Lactate Dehydrogenase - metabolism
Lactate dehydrogenase
Lactic acid
Mechanical stimuli
Morphology
Perfusion
Pituitary (anterior)
Placenta
Pregnancy
Scanning electron microscopy
Shear stress
Trophoblasts - cytology
Trophoblasts - metabolism
Veins & arteries
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Summary:During pregnancy, freely floating placental villi are adapted to fluid shear stress due to placental perfusion with maternal plasma and blood. In vitro culture of placental villous explants is widely performed under static conditions, hoping the conditions may represent the in utero environment. However, static placental villous explant culture dramatically differs from the in vivo situation. Thus, we established a flow culture system for placental villous explants and compared commonly used static cultured tissue to flow cultured tissue using transmission and scanning electron microscopy, immunohistochemistry, and lactate dehydrogenase (LDH) and human chorionic gonadotropin (hCG) measurements. The data revealed a better structural and biochemical integrity of flow cultured tissue compared to static cultured tissue. Thus, this new flow system can be used to simulate the blood flow from the mother to the placenta and back in the most native-like in vitro system so far and thus can enable novel study designs.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22147464