Biosynthesis and Antimicrobial Activity of Pseudodesmin and Viscosinamide Cyclic Lipopeptides Produced by Pseudomonads Associated with the Cocoyam Rhizosphere

Pseudomonas cyclic lipopeptides (CLPs) are encoded non-ribosomally by biosynthetic gene clusters (BGCs) and possess diverse biological activities. In this study, we conducted chemical structure and BGC analyses with antimicrobial activity assays for two CLPs produced by Pseudomonas strains isolated...

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Published in:Microorganisms (Basel) 2020-07, Vol.8 (7), p.1079
Main Authors: Oni, Feyisara E., Geudens, Niels, Adiobo, Amayana, Omoboye, Olumide O., Enow, Elsie A., Onyeka, Joseph T., Salami, Ayodeji E., De Mot, René, Martins, José C., Höfte, Monica
Format: Article
Language:English
Subjects:
Amino acids
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
Biosynthesis
CLP perception
defense response
Disintegration
Divergence
evolution
Fatty acids
Gene clusters
Genes
Genomes
Lipopeptides
Lysis
NMR
Nuclear magnetic resonance
Pathogens
Peptides
Pseudomonas
Pythium myriotylum
R&D
Research & development
Rhizosphere
Soils
viscosin group
Viscosinamide
WLIP
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Summary:Pseudomonas cyclic lipopeptides (CLPs) are encoded non-ribosomally by biosynthetic gene clusters (BGCs) and possess diverse biological activities. In this study, we conducted chemical structure and BGC analyses with antimicrobial activity assays for two CLPs produced by Pseudomonas strains isolated from the cocoyam rhizosphere in Cameroon and Nigeria. LC-MS and NMR analyses showed that the Pseudomonas sp. COR52 and A2W4.9 produce pseudodesmin and viscosinamide, respectively. These CLPs belong to the Viscosin group characterized by a nonapeptidic moiety with a 7-membered macrocycle. Similar to other Viscosin-group CLPs, the initiatory non-ribosomal peptide synthetase (NRPS) gene of the viscosinamide BGC is situated remotely from the other two NRPS genes. In contrast, the pseudodesmin genes are all clustered in a single genomic locus. Nano- to micromolar levels of pseudodesmin and viscosinamide led to the hyphal distortion and/or disintegration of Rhizoctonia solani AG2-2 and Pythium myriotylum CMR1, whereas similar levels of White Line-Inducing Principle (WLIP), another member of the Viscosin group, resulted in complete lysis of both soil-borne phytopathogens. In addition to the identification of the biosynthetic genes of these two CLPs and the demonstration of their interaction with soil-borne pathogens, this study provides further insights regarding evolutionary divergence within the Viscosin group.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms8071079